ATF3 antibody (AA 113-130)

Catalog No. ABIN401262

Product Details anti-ATF3 Antibody

Target: ATF3 (Activating Transcription Factor 3 (ATF3))

Binding Specificity: AA 113-130

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATF3 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody is directed against ATF3 and is useful in determining its presence in various assays including ELISA and western blotting. It recognizes over-expressed ATF3 protein found in various expression systems.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity chromatography

Isotype: IgG

Application Details

Application Notes: ELISA: 1: 5,000 to 1: 20,000. Western blot: 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for ATF3

Target: ATF3 (Activating Transcription Factor 3 (ATF3))

Alternative Name: ATF3 (ATF3 Products)

Synonyms: zgc:55526 antibody, ATF3 antibody, LRG-21 antibody, LRF-1 antibody, LRFI antibody, atf-3 antibody, activating transcription factor 3 antibody, activating transcription factor 3 L homeolog antibody, atf3 antibody, ATF3 antibody, Atf3 antibody, atf3.L antibody

Background: ATF3, or Activating Transcription Factor 3, is a member of mammalian activation TF/CREB protein family of transcription factors. ATF3 binds the cAMP response element (cre) (consensus: 5'-gtgacgt[ac][ag]-3'), a sequence present in many viral and cellular promoters. However, ATF3 represses rather than activates transcription from promoters with ATF sites stabilizing inhibitory co-factors at the promoter. Alternate splicing forms of ATF3, called ATF3 delta Zip, lack the leucine zipper domain and do not bind DNA. ATF3 delta Zip stimulates transcription, presumably by sequestering inhibitory co-factors away from the promoter. Human ATF3 is a 20575 Da protein composed of 181 amino acids.Synonyms: ATF-3, Activating transcription factor 3, Cyclic AMP-dependent transcription factor ATF-3, cAMP-dependent transcription factor ATF-3

Gene ID: 467

NCBI Accession: NP_001025458

UniProt: P18847

Pathways: Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Unfolded Protein Response