ATF3 antibody (AA 113-130)
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- Target See all ATF3 Antibodies
- ATF3 (Activating Transcription Factor 3 (ATF3))
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Binding Specificity
- AA 113-130
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This ATF3 antibody is un-conjugated
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Specificity
- This antibody is directed against ATF3 and is useful in determining its presence in various assays including ELISA and western blotting. It recognizes over-expressed ATF3 protein found in various expression systems.
- Cross-Reactivity (Details)
- Species reactivity (tested):Human.
- Purification
- Affinity chromatography
- Isotype
- IgG
- Top Product
- Discover our top product ATF3 Primary Antibody
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- Application Notes
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ELISA: 1: 5,000 to 1: 20,000. Western blot: 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL (by UV absorbance at 280 nm)
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
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- Target
- ATF3 (Activating Transcription Factor 3 (ATF3))
- Alternative Name
- ATF3 (ATF3 Products)
- Synonyms
- zgc:55526 antibody, ATF3 antibody, LRG-21 antibody, LRF-1 antibody, LRFI antibody, atf-3 antibody, activating transcription factor 3 antibody, activating transcription factor 3 L homeolog antibody, atf3 antibody, ATF3 antibody, Atf3 antibody, atf3.L antibody
- Background
- ATF3, or Activating Transcription Factor 3, is a member of mammalian activation TF/CREB protein family of transcription factors. ATF3 binds the cAMP response element (cre) (consensus: 5'-gtgacgt[ac][ag]-3'), a sequence present in many viral and cellular promoters. However, ATF3 represses rather than activates transcription from promoters with ATF sites stabilizing inhibitory co-factors at the promoter. Alternate splicing forms of ATF3, called ATF3 delta Zip, lack the leucine zipper domain and do not bind DNA. ATF3 delta Zip stimulates transcription, presumably by sequestering inhibitory co-factors away from the promoter. Human ATF3 is a 20575 Da protein composed of 181 amino acids.Synonyms: ATF-3, Activating transcription factor 3, Cyclic AMP-dependent transcription factor ATF-3, cAMP-dependent transcription factor ATF-3
- Gene ID
- 467
- NCBI Accession
- NP_001025458
- UniProt
- P18847
- Pathways
- Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Unfolded Protein Response
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