RAD54L antibody (N-Term)

Catalog No. ABIN401275

Product Details anti-RAD54L Antibody

Target: RAD54L (RAD54-Like (RAD54L))

Binding Specificity: AA 1-17, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD54L antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: BLAST analysis indicates 100 % homology of the immunizing sequence with RAD54 from Human. Cross reactivity with RAD54 protein homologues from other sources may not occur as sequence homology varies by at least one amino acid residue in this sequence.

Purification: Immunoaffinity Chromatography using peptide coupled to agarose beads.

Immunogen: Synthetic peptide corresponding aa 1-17 of Human RAD54 protein.

Isotype: IgG

Application Details

Application Notes: Suitable for use in ELISA and by Western blot. Expect a band approximately 84 kDa in size corresponding to RAD54 protein by Westernblotting in the appropriate cell lysate or extract. Splice variants exist for this protein thatmay result in the detection of lower molecular weight bands. Recommended Dilutions: ELISA: 1/10000-1/40000. Western Blot: 1/500-1/2000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for RAD54L

Target: RAD54L (RAD54-Like (RAD54L))

Alternative Name: RAD54L (RAD54L Products)

Synonyms: HR54 antibody, RAD54A antibody, hHR54 antibody, hRAD54 antibody, RAD54 antibody, zgc:56289 antibody, rad54l antibody, MGC69368 antibody, GdRAD54 antibody, RAD54-like antibody, RAD54L antibody, RAD54 like antibody, RAD54 like (S. cerevisiae) antibody, RAD54-like (S. cerevisiae) antibody, RAD54L antibody, Rad54l antibody, rad54l antibody

Background: RAD54, also known as hHR54, HR54, hRAD54 and RAD54A, belongs to the DEAD-like helicase superfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologous recombination and repair of DNA. This protein has been shown to play a role in homologous recombination related repair of DNA double-strand breaks. The binding of this protein to double-strand DNA induces a DNA topological change, which is thought to facilitate homologous DNA pairing, and stimulate DNA recombination.Synonyms: DNA repair and recombination protein RAD54-like, RAD54 homolog, RAD54A, hHR54, hRAD54

Gene ID: 8438

NCBI Accession: NP_001136020

UniProt: Q92698

Pathways: DNA Damage Repair