SLIT2 antibody (AA 484-500)

Catalog No. ABIN401286

Product Details anti-SLIT2 Antibody

Target: SLIT2 (Slit Homolog 2 (Drosophila) (SLIT2))

Binding Specificity: AA 484-500

Reactivity: Human, Rat, Mouse, Chicken, Cow, Tetraodon nigroviridis, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLIT2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody is directed against SLIT-2 protein.

Cross-Reactivity (Details): Species reactivity (expected):Chicken, Mouse, Rat, Bovine, Xenopus laevis.
Species reactivity (tested):Human.

Purification: Affinity Chromatography

Immunogen: Synthetic peptide corresponding aa 484-500 of Human SLIT-2 protein.

Isotype: IgG

Application Details

Application Notes: ELISA: 1/1,500 - 1/6,000. Western Blot: 1/500 - 1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted 2-8 °C fo one month or (in aliquots) at-20 °C for longer.

Target Details for SLIT2

Target: SLIT2 (Slit Homolog 2 (Drosophila) (SLIT2))

Alternative Name: SLIT2 (SLIT2 Products)

Synonyms: SLIL3 antibody, Slit-2 antibody, Drad-1 antibody, E030015M03Rik antibody, E130320P19Rik antibody, Slil3 antibody, mKIAA4141 antibody, Slit antibody, slil3 antibody, slit-2 antibody, slit2-a antibody, SLIT2 antibody, slit guidance ligand 2 antibody, slit homolog 2 (Drosophila) antibody, slit guidance ligand 2 S homeolog antibody, SLIT2 antibody, Slit2 antibody, slit2 antibody, slit2.S antibody

Background: SLIT-2 is thought to act as a molecular guidance cue in cellular migration, and its function appears to be mediated by interaction with roundabout homologue receptors. SLIT-1 and SLIT-2 seem to be essential for midline guidance in the forebrain by acting as repulsive signals preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development SLIT-2 may play role in guiding commissural axons once they reach the floor plate by modulating the response to netrin. In vitro, SLIT-2 silences the attractive effect of NTN1 but not its growthstimulatory effect and silencing requires the formation of a ROBO1-DCC complex. In vitro, only commissural axons that crossed the midline responded to SLIT-2. In the developing visual system this protein functions as a repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. SLIT-2 is a secreted protein predominantly expressed in fetal lung and kidney, and adult spinal cord. Weak expression is observed in adult adrenal gland, thyroid, trachea and other tissues examined. Multiple isoforms have been reported for this product.Synonyms: SLIL3, SLIT-2, Slit homolog 2 protein

Gene ID: 9353

NCBI Accession: NP_004778

UniProt: O94813

Pathways: Regulation of Actin Filament Polymerization, Regulation of Cell Size, Smooth Muscle Cell Migration