CDK9 antibody (pThr29)

Catalog No. ABIN401390

The Rabbit Polyclonal anti-CDK9 antibody is suitable to detect CDK9 in samples from Human, Mouse and Rat. It has been validated for WB, IP and EIA.

Quick Overview for CDK9 antibody (pThr29) (ABIN401390)

Target: CDK9 (Cyclin-Dependent Kinase 9 (CDK9))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK9 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Product Details anti-CDK9 Antibody

Binding Specificity: pThr29

Specificity: This antibody is specific for CDK9 protein phosphorylated at T29. Expect a band approximately 42 kDa in size corresponding to phosphorylated CDK9 protein by Western blotting in the appropriate cell lysate or extract.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography

Immunogen: Synthetic peptide corresponding to residues surrounding Thr 29 in the Human CDK9 protein.

Isotype: IgG

Application Details

Application Notes: ELISA: 1/5,000-1/24,000. Western Blot: 1/200-1/2,000. Immunoprecipitation: 1/100.

Restrictions: For Research Use only

Handling

Concentration: 1.3 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at-20 °C.

Target Details for CDK9

Target: CDK9 (Cyclin-Dependent Kinase 9 (CDK9))

Alternative Name: CDK9

Background: CDK9 (PITALRE) (also known as cyclin-dependent kinase 9, Serine/threonine-protein kinase PITALRE, C-2K and Cell division cycle 2-like protein kinase 4) is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2 and are known as important cell cycle regulators. CDK9 (PITALRE) interacts with a conserved domain in the TRAF-C region of the tumor necrosis factor signal transducer TRAF2. This kinase was also found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase IIdirected transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with, and is regulated by, its regulatory subunit, cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggests a possible involvement of this protein in AIDS. Tat stimulates human HIV-1 viral transcription elongation. This suggests that cyclin T1/CDK9(PITALRE) is one of the HIV-1 required host cellular cofactors generated during T cell activation. Cyclin T1/CDK9(PITALRE) has been shown to interact with Tat to restore Tat activation in HeLa nuclear extracts depleted of P-TEFb. CDK9(PITALRE) activity and cyclin T1 are essential for activation of viral transcription when tethered to the heterologous Rev response element RNA via the regulator of expression of virion Rev. CDK9 (PITALRE) is a ubiquitously expressed nuclear protein. CDK9 kinase activity is regulated by autophosphoryaltion of CDK9 at T29 and T186. T29 phosphorylation is uniquely induced by the HTLV-1 viral oncoprotein Tax.Synonyms: CDC2L4, Cell division cycle 2-like protein kinase 4, Cell division protein kinase 9, Cyclin-dependent kinase 9, PITALRE, Serine/threonine-protein kinase PITALRE

Gene ID: 1025

NCBI Accession: NP_001252

UniProt: P50750

Pathways: Cell Division Cycle