SMAD1 antibody (Ser206)
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- Target See all SMAD1 Antibodies
- SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))
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Binding Specificity
- Ser206
- Reactivity
- Human, Mouse, Rat, Cow, Dog, Chicken, Horse, Drosophila melanogaster, Sheep, Pig, Bonobo, Chimpanzee, Duckbill (Platypus), Gorilla, Macaque, Opossum, Orang-Utan, Salmon
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This SMAD1 antibody is un-conjugated
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Specificity
- This antibody is directed against SMAD1 protein.
- Cross-Reactivity (Details)
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Species reactivity (expected):Duckbill platypus, Drosophila, Mouse, Opossum, Chimpanzee, Bonobo, Orangutan, Gorilla, Macaque, Cattle, Sheep, Porcine, Rat, Horse, Salmon, Chicken, Canine.
Species reactivity (tested):Human. - Purification
- Affinity chromatography
- Immunogen
- Synthetic peptide corresponding to the region of amino acids containing serine 206 of human SMAD1 protein
- Isotype
- IgG
- Top Product
- Discover our top product SMAD1 Primary Antibody
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- Application Notes
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ELISA: 1/25,000 - 1/30,000. Western Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.31 mg/mL (by UV absorbance at 280 nm)
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- -20 °C
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- Target
- SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))
- Alternative Name
- SMAD1 (SMAD1 Products)
- Synonyms
- MLP antibody, Xmad1 antibody, Xsmad1 antibody, bsp1 antibody, jv4-1 antibody, jv41 antibody, madh1 antibody, madr1 antibody, smad-1 antibody, MADH1 antibody, fb39h09 antibody, wu:fb39h09 antibody, wu:fb97e04 antibody, 2/23 antibody, CG12399 antibody, Dmel\\CG12399 antibody, E(zen)2 antibody, En(vvl) antibody, MAD antibody, Mat antibody, P-Mad antibody, P-mad antibody, PMad antibody, Smad antibody, Smad1 antibody, apg antibody, c28 antibody, dMAD antibody, dMad antibody, l(2)K00237 antibody, l(2)k00237 antibody, mad antibody, p-Mad antibody, pMAD antibody, pMad antibody, pSmad antibody, BSP-1 antibody, BSP1 antibody, JV4-1 antibody, JV41 antibody, MADR1 antibody, AI528653 antibody, Madh1 antibody, Madr1 antibody, Mlp1 antibody, MusMLP antibody, smad1 antibody, xmad antibody, SMAD family member 1 antibody, Mothers against dpp antibody, SMAD family member 1 L homeolog antibody, smad1 antibody, SMAD1 antibody, Mad antibody, Smad1 antibody, smad1.L antibody
- Background
- SMAD1 is also known Mothers Against Decapentaplegic homolog 1, Mothers against DPP homolog 1, hSMAD-3, JV4-1, Transforming growth factor-Beta-Signaling Protein 1 or BSP1. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. SMAD1, as a transcriptional modulator, is activated by BMP (Bone Morphogenetic Protein) type 1 receptor kinase (it is a receptor-regulated SMAD or R-SMAD). BMPs are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. SMAD proteins have been implicated as downstream effectors of TGF beta/BMP signaling. In response to BMP ligands, SMAD1 can be phosphorylated (other sites besides the most prominent of S206, are S187, S195, and S214). S-206 is phosphorylated by ERK in response to mitogenic growth factors, or by recombinant ERK in vitro, this can be tested by treating cells with EGF or in cancer cells where Ras is activated. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is also a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasomemediated degradation.Synonyms: BSP-1, BSP1, JV4-1, MAD homolog 1, MADH1, MADR1, Mad-related protein 1, Mothers against DPP homolog 1, Mothers against decapentaplegic homolog 1, SMAD 1, SMAD family member 1, SMAD-1, Transforming growth factor-beta-signaling protein 1
- Gene ID
- 4086
- NCBI Accession
- NP_001003688
- UniProt
- Q15797
- Pathways
- Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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