RRM2B antibody (N-Term)

Catalog No. ABIN401481

The Rabbit Polyclonal anti-RRM2B antibody (ABIN401481) specifically detects RRM2B in WB and EIA. The antibody is reactive with Human samples.

Quick Overview for RRM2B antibody (N-Term) (ABIN401481)

Target: RRM2B (Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RRM2B antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-RRM2B Antibody

Binding Specificity: N-Term

Specificity: This antibody is directed against RRM2B.

Cross-Reactivity (Details): Species reactivity (expected):Chimpanzee, Orangutan, Macaque, Porcine, Drosophila.
Species reactivity (tested):Human.

Purification: Affinity Purified

Immunogen: Synthetic peptide corresponding to a region near the N-terminus of human RRM2B1 protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1/3,000. Western Blot: 1,0 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.82 mg/mL (by UV absorbance at 280nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for RRM2B

Target: RRM2B (Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B))

Alternative Name: RRM2B / P53R2

Background: RRM2B/p53-R2, or p53-inducible ribonucleotide reductase small subunit 2-like protein, is a member of a broad superfamily of ferritin-like di-ironcarboxylate proteins. The RRM2B protein is an enzyme that catalyzes the conversion of ribonucleotides to deoxyribonucleotides that are essential for DNA synthesis, and is found in all eukaryotes. RRM2B plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. It supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. It contains an iron-tyrosyl free radical center required for catalysis, and forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage. It is a heterotetramer with a large (RRM1) subunit, and interacts with p53/TP53. The interaction with RRM1 occurs in response to DNA damage and results in its translocation from cytoplasm to nucleus. It is widely expressed at a high level in skeletal muscle and at a weak level in thymus, and expressed in epithelial dysplasias and squamous cell carcinoma. Defects in RRM2B are the cause of encephalomyopathic mitochondrial depletion syndrome with renal tubulopathy (EMDSRT). Mitochondrial DNA depletion syndrome (MDS) is a clinically heterogeneous group of disorders characterized by a reduction in mitochondrial DNA (mtDNA) copy number. The encephalomyopathic form with renal tubulopathy is presented with various combinations of hypotonia, tubulopathy, seizures, respiratory distress, diarrhea, and lactic acidosis.Synonyms: Ribonucleoside-diphosphate reductase subunit M2 B, TP53-inducible ribonucleotide reductase M2 B, p53-inducible ribonucleotide reductase small subunit 2-like protein

Gene ID: 50484

NCBI Accession: NP_001165948

UniProt: Q7LG56

Pathways: p53 Signaling, Negative Regulation of intrinsic apoptotic Signaling