RRM2B antibody (N-Term)

Catalog No. ABIN500437

This Rabbit Polyclonal antibody specifically detects RRM2B in WB, IHC (p) and EIA. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for RRM2B antibody (N-Term) (ABIN500437)

Target: RRM2B (Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RRM2B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-RRM2B Antibody

Binding Specificity: AA 2-17, N-Term

Specificity: This antibody detects p53R2 at N-term.

Purification: Peptide Affinity Chromatography

Immunogen: p53R2 antibody was raised against a synthetic peptide corresponding to amino acids 2 to 17 of human p53R2.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 0.5-1 μg/mL (A 39 kDa band can be detected). Immunohistochemistry on Paraffin Sections. Positive Controls: A431 Cell Lysate, 3T3 Whole Cell Lysate, Human Lung Tissue Lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for RRM2B

Target: RRM2B (Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B))

Alternative Name: RRM2B / P53R2

Background: The p53 tumor-suppresspr gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including p53R2 , Chk2 (2), p53AIP1 (3), Noxa (4), PIDD (5), and PID/MTA2 (6), were recently discovered. p53R2 is a p53 inducible gene that contains a p53 binding sequence and encodes a subunit of the enzyme ribonucleotide reductase (1,7). p53R2 is induced by the reagents, ultraviolet and g-irradiation that cause DNA damages. The product of p53R2 gene is directly involved in the p53 checkpoint for repair of damaged DNA. The isoform of the p53 family member p73 also induces p53R2 expression (8). p53R2 is an important target of p53 for tumour suppression.Synonyms: Ribonucleoside-diphosphate reductase subunit M2 B, TP53-inducible ribonucleotide reductase M2 B, p53-inducible ribonucleotide reductase small subunit 2-like protein

Gene ID: 50484

UniProt: Q7LG56

Pathways: p53 Signaling, Negative Regulation of intrinsic apoptotic Signaling