COX IV antibody (C-Term, Isoform 1, Isoform 2)

Catalog No. ABIN401531

Product Details anti-COX IV Antibody

Target: COX IV (COX4I1) (Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1))

Binding Specificity: C-Term, Isoform 1, Isoform 2

Reactivity: Human, Rat, Mouse, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This COX IV antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: c-RWDYEKKQWK K

Specificity: This antibody detetcs COX4I2/COX4I1 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, DogSpecies reactivity (tested):Human
Also reacts to: Isoform 1

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the C Terminus of the protein sequence according to NP_115998.2 . Genename: COX4I2

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1: 2000. Western blot: 1-3 μg/mL. Approx 17 kDa band observed in Human Skeletal Muscle lysates(calculated MW of 19.7 kDa according to NP_115998.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3, 0.02 % sodium azide, 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for COX IV

Target: COX IV (COX4I1) (Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1))

Alternative Name: COX IV (COX4I1 Products)

Synonyms: COX4 antibody, COX4-1 antibody, COXIV antibody, mg:bb02d03 antibody, zgc:110058 antibody, GB20012 antibody, AL024441 antibody, Cox4 antibody, Cox4a antibody, cox4 antibody, cox4i1 antibody, coxiv antibody, BcDNA:AT07685 antibody, CG10396 antibody, COX IV antibody, CX41 antibody, CX42 antibody, Dmel\CG10396 antibody, IV antibody, cytochrome c oxidase subunit 4I1 antibody, cytochrome c oxidase subunit IV isoform 1 antibody, cytochrome c oxidase subunit 4 isoform 1, mitochondrial antibody, cytochrome c oxidase polypeptide IV antibody, cytochrome c oxidase subunit IV antibody, cytochrome c oxidase subunit 4i1 antibody, cytochrome c oxidase subunit IV isoform 1 S homeolog antibody, Cytochrome c oxidase subunit 4-like antibody, COX4I1 antibody, cox4i1 antibody, LOC412396 antibody, Cox4I1 antibody, LOC780848 antibody, BMEII0322 antibody, RSal33209_1582 antibody, SJAG_00976 antibody, HMPREF0733_11926 antibody, Cox4i1 antibody, cox4i1.S antibody, COX4L antibody

Background: Cytochrome C Oxidase (COX), which is located in the inner mitochondrial membrane, is the terminal enzyme complex of the mitochondrial electron transport chain. It collects electrons that are transferred from reduced CYTOCHROME C and donates them to molecular oxygen, which is then reduced to water. It is composed of CYTOCHROME A and CYTOCHROME B, two copper atoms, and 13 different protein subunits, three of which are encoded by the mitochondrial DNA and ten others by nuclear DNA (mammals).Synonyms: COX4I2, COX4L2, Cytochrome c oxidase subunit IV isoform 2, mitochondrial Cytochrome c oxidase subunit 4 isoform 2

Gene ID: 84701

NCBI Accession: NP_115998

UniProt: Q96KJ9

Pathways: Proton Transport