MEF2A antibody (pThr319)

Catalog No. ABIN401543

This Rabbit Polyclonal antibody specifically detects MEF2A in IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MEF2A antibody (pThr319) (ABIN401543)

Target: MEF2A (Myocyte Enhancer Factor 2A (MEF2A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MEF2A antibody is un-conjugated

Application: Immunofluorescence (IF)

Product Details anti-MEF2A Antibody

Binding Specificity: pThr319

Specificity: This antibody detects endogenous levels of MEF2A only when phosphorylated at Thr319.

Purification: Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from Human MEF2A around the phosphorylation site of Thr319 (V-T-TP-P-S).

Application Details

Application Notes: Immunofluorescence: 1/100approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at-20 °C.

Target Details for MEF2A

Target: MEF2A (Myocyte Enhancer Factor 2A (MEF2A))

Alternative Name: MEF2A

Background: The myocyte enhancer factor-2 (MEF-2) family of transcription factors associated with co-repessors or co-activators to regulate development and function of T cells, neuronal cells and muscle cells. Four family members arise from alternatively spliced transcripts, termed MEF-2A, -2B, -2C and -2D. These members bind as homo- and heterodimers to the MEF-2 site in the promoter region of affected genes. Differential regulation in the expression of the four transcripts implies functional distinction for each during embryogenesis and development. The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression. The myogenic basic helix-loop-helix proteins, including MyoD, myogenin, Myf-5 and MRF-4, are one class of identified factors. A second family of DNA binding regulatory proteins is the myocyte-specific enhancer factor-2 (MEF-2) family. Each of these proteins binds to the MEF-2 target DNA sequence present in the regulatory regions of many muscle-specific genes.Synonyms: MEF2, Myocyte-specific enhancer factor 2A, Serum response factor-like protein 1

Gene ID: 4205

NCBI Accession: NP_001124398

UniProt: Q02078

Pathways: Neurotrophin Signaling Pathway, Activation of Innate immune Response, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades