High Mobility Group Nucleosome Binding Domain 1 (HMGN1) (Middle Region) antibody

Details for Product No. ABIN404814
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Antigen
Synonyms HMG14, HMG-14, Hmg14, hmg14, hmgn1, hmgn1b
Epitope
Middle Region
(17), (16), (5), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Rabbit, Rat (Rattus), Mouse (Murine), Cow (Bovine), Dog (Canine), Human
(49), (38), (37), (28), (27), (26), (26), (2), (1), (1)
Host
Rabbit
(50), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(25), (21), (20), (16), (12), (8), (5), (2), (1), (1), (1)
Pubmed 1 reference available
Catalog no. ABIN404814
Quantity 50 µg
Price
289.00 $   Plus shipping costs $45.00
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Immunogen Synthetic peptide directed towards the middle region of human HMGN1
Sequence PKKAAAKDKSSDKKVQTKGKRGAKGKQAEVANQETKEDLP AENGETKTEE
Predicted Reactivity Bovine : 100 %, Human : 100 %, Rabbit : 90 %, Rat : 80 %, Mouse : 70 % Application WB, IHC
Characteristics This is a rabbit polyclonal antibody against HMGN1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name HMGN1
Background Chromosomal protein HMG14 (HMGN1) and its close analog HMG17 (MIM 163910) bind to the inner side of the nucleosomal DNA, potentially altering the interaction between the DNA and the histone octamer. The 2 proteins may be involved in the process that maintains transcribable genes in a unique chromatin conformation. Their ubiquitous distribution and relative abundance, as well as the high evolutionary conservation of the DNA-binding domain of the HMG14 family of proteins, suggest that they may be involved in an important cellular function.Chromosomal protein HMG14 and its close analog HMG17 (MIM 163910) bind to the inner side of the nucleosomal DNA, potentially altering the interaction between the DNA and the histone octamer. The 2 proteins may be involved in the process that maintains transcribable genes in a unique chromatin conformation. Their ubiquitous distribution and relative abundance, as well as the high evolutionary conservation of the DNA-binding domain of the HMG14 family of proteins, suggest that they may be involved in an important cellular function.
Molecular Weight 11 kDa
Gene ID 3150
NCBI Accession NM_004965, NP_004956
UniProt P05114
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 100 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-High Mobility Group Nucleosome Binding Domain 1 (HMGN1) (Middle Region) antibody WB Suggested Anti-HMGN1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: 721_B cell lysate
anti-High Mobility Group Nucleosome Binding Domain 1 (HMGN1) (Middle Region) antibody (2) anti-High Mobility Group Nucleosome Binding Domain 1 (HMGN1) (Middle Region) antibody (Image 2)
Background publications Zhu, Hansen: "HMGN1 modulates estrogen-mediated transcriptional activation through interactions with specific DNA-binding transcription factors." in: Molecular and cellular biology, Vol. 27, Issue 24, pp. 8859-73, 2007 (PubMed).

Hosts (50), (1)
Reactivities (49), (38), (37), (28), (27), (26), (26), (2), (1), (1)
Applications (25), (21), (20), (16), (12), (8), (5), (2), (1), (1), (1)
Conjugates (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Epitopes (17), (16), (5), (3), (2), (1), (1), (1), (1), (1), (1), (1)
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