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HMGN1 antibody (High Mobility Group Nucleosome Binding Domain 1) (AA 36-85) Primary Antibody

HMGN1 Reactivity: Human, Mouse, Rat, Cow, Horse, Monkey IHC, IHC (p), WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6741544
$727.83
Plus shipping costs $45.00
100 μL
local_shipping Shipping to: United States
Delivery in 11 to 14 Business Days
  • Target
    HMGN1
    Binding Specificity
    • 17
    • 16
    • 15
    • 11
    • 8
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 36-85
    Reactivity
    • 64
    • 13
    • 11
    • 8
    • 6
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Cow, Horse, Monkey
    Host
    • 66
    Rabbit
    Clonality
    • 66
    Polyclonal
    Conjugate
    • 28
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    This HMGN1 antibody is un-conjugated
    Application
    • 38
    • 30
    • 26
    • 22
    • 22
    • 15
    • 9
    • 6
    • 2
    • 2
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    Brand
    IHC-plus™
    Specificity
    Human HMGN1
    Predicted Reactivity
    Percent identity by BLAST analysis: Human, Bovine (100%) Rabbit (92%) Rat (84%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic peptide located between aa36-85 of human HMGN1 (P05114, NP_004956). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Baboon, Monkey, Bovine, Horse, Armadillo (100%), Gibbon, Rabbit (92%), Opossum (91%), Platypus (86%), Marmoset, Pig (85%), Rat (84%), Mouse (76%).

    Type of Immunogen: Synthetic peptide
    Isotype
    IgG
  • Application Notes
    Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.
    Comment

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Distilled water
    Concentration
    Lot specific
    Buffer
    Lyophilized from PBS with 2 % sucrose
    Handling Advice
    Avoid repeat freeze-thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target
    HMGN1
    Alternative Name
    HMGN1 (HMGN1 Antibody Abstract)
    Synonyms
    HMG14, hmg14, hmgn1, hmgn1b, HMG-14, Hmg14, high mobility group nucleosome binding domain 1, high mobility group nucleosome binding domain 1 S homeolog, high mobility group nucleosomal binding domain 1, non-histone chromosomal protein HMG-14, HMGN1, hmgn1.S, hmgn1, Hmgn1, LOC785479
    Background
    Name/Gene ID: HMGN1

    Synonyms: HMGN1, HMG14
    Gene ID
    3150
    NCBI Accession
    NP_004956
    UniProt
    P05114
    Pathways
    Chromatin Binding
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