Hypoxia Inducible Factor 1, alpha Subunit Inhibitor (HIF1AN) (Middle Region) antibody

Details for Product No. ABIN404835
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Antigen
Synonyms HIF-1a, HIF1a, hif-1a, hif1a, FIH1, 2310046M24Rik, A830014H24Rik, cb728, id:ibd5139
Epitope
Middle Region
(16), (14), (3), (2), (2), (2), (2), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine), Xenopus laevis, Human, Zebrafish (Danio rerio)
(88), (27), (25), (14), (14), (12), (12), (1), (1)
Host
Rabbit
(58), (29)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(74), (29), (19), (17), (11), (11), (10), (2), (2), (2), (1), (1), (1)
Pubmed 1 reference available
Quantity 50 μg
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Catalog No. ABIN404835
289.00 $
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Immunogen Synthetic peptide directed towards the middle region of human HIF1AN
Sequence TSNLLLIGME GNVTPAHYDE QQNFFAQIKG YKRCILFPPD QFECLYPYPV
Predicted Reactivity Bovine : 100 %, Chicken : 100 %, Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Rabbit : 100 %, Rat : 100 %, Zebrafish : 100 %, African clawed frog : 90 %
Characteristics This is a rabbit polyclonal antibody against HIF1AN. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name HIF1AN
Background HIF1AN is a co-repressor that interacts with hypoxia-inducible factor 1 (HIF-1) alpha and the von Hippel-Lindau tumor suppressor protein to mediate repression of HIF-1 transcriptional activity.
Molecular Weight 40 kDa
Gene ID 55662
NCBI Accession NP_060372, NM_017902
UniProt Q9NWT6
Research Area Cancer, Transcription Factors
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 349 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Background publications Linke, Stojkoski, Kewley et al.: "Substrate requirements of the oxygen-sensing asparaginyl hydroxylase factor-inhibiting hypoxia-inducible factor." in: The Journal of biological chemistry, Vol. 279, Issue 14, pp. 14391-7, 2004 (PubMed).

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