Claudin 18 antibody (Middle Region)

Catalog No. ABIN404887

This Rabbit Polyclonal antibody specifically detects Claudin 18 in WB. It exhibits reactivity toward Human, Rat, Mouse, Cow, Dog, Horse, Rabbit and Guinea Pig.

Quick Overview for Claudin 18 antibody (Middle Region) (ABIN404887)

Target: Claudin 18 (CLDN18)

Reactivity: Human, Rat, Mouse, Cow, Dog, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Claudin 18 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Claudin 18 Antibody

Binding Specificity: Middle Region

Purpose: CLDN18 Antibody - middle region

Sequence: YHASGHSVAY KPGGFKASTG FGSNTKNKKI YDGGARTEDE VQSYPSKHDY

Predicted Reactivity: Cow: 79%, Dog: 79%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 86%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against CLDN18. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CLDN18

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP33612-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Claudin 18

Target: Claudin 18 (CLDN18)

Alternative Name: CLDN18

Background:

Background Information: CLDN18 plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.CLDN18 belongs to the large claudin family of proteins, which form tight junction strands in epithelial cells (Niimi et al., 2001 [PubMed 11585919]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-282 AY358479.1 28-309 283-1864 AK098474.1 274-1855 1865-2307 BM785703.1 143-585 2308-2868 AK098474.1 2297-2857 2869-3359 AY102073.1 394-884

Gene Name: Claudin 18

Alternative Symbols: SFTA5, SFTPJ

Protein Name: Claudin-18

Molecular Weight: 28kDa

Gene ID: 51208

NCBI Accession: NP_057453

UniProt: P56856

Pathways: Cell-Cell Junction Organization, Hepatitis C