Mediator Complex Subunit 6 (MED6) (Middle Region) antibody

Details for Product No. ABIN404928
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Antigen
Synonyms NY-REN-28, wu:fa14c11, zgc:86933, 1500012F11Rik, AU019660, AV213303
Epitope
Middle Region
(8), (8), (8), (8), (5), (2), (2), (1), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine), Human, Chicken
(36), (4), (3), (1), (1), (1)
Host
Rabbit
(29), (7)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)
(33), (22), (4), (2), (1)
Pubmed 1 reference available
Quantity 50 μg
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Catalog No. ABIN404928
289.00 $
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Immunogen Synthetic peptide directed towards the middle region of human MED6
Sequence LLLDLRQKFP PKFVQLKPGE KPVPVDQTKK EAEPIPETVK PEEKETTKNV
Predicted Reactivity Dog : 100 %, Human : 100 %, Bovine : 90 %, Pig : 90 %, Rabbit : 90 %, Chicken : 80 %, Mouse : 80 %, Rat : 80 %
Characteristics This is a rabbit polyclonal antibody against MED6. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name MED6
Background MED6 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. And mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Molecular Weight 28 kDa
Gene ID 10001
NCBI Accession NM_005466, NP_005457
UniProt O75586
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 246 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Mediator Complex Subunit 6 (MED6) (Middle Region) antibody WB Suggested Anti-MED6 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: 721_B cell lysate
Background publications Pavri, Lewis, Kim et al.: "PARP-1 determines specificity in a retinoid signaling pathway via direct modulation of mediator." in: Molecular cell, Vol. 18, Issue 1, pp. 83-96, 2005 (PubMed).

Validation Images
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