Ribosomal Protein L3 (RPL3) (N-Term) antibody

Details for Product No. ABIN405972
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Antigen
Synonyms CG4863, Dmel\\CG4863, L3, Rpl3, anon-EST:GressD4, rpL3, wu:fa99g02, wu:fb65e09, zgc:110350, ASC-1, TARBP-B, F2, J1
Epitope
N-Term
(12), (7), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio), Cow (Bovine), Human, Chicken
(36), (15), (15), (1), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(40)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1)
Application
Western Blotting (WB)
(40), (26), (25), (6), (6), (2)
Pubmed 1 reference available
Quantity 50 µg
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Catalog No. ABIN405972
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Immunogen Synthetic peptide directed towards the N terminal of human RPL3
Sequence DPSKPVHLTA FLGYKAGMTH IVREVDRPGS KVNKKEVVEA VTIVETPPMV
Predicted Reactivity African clawed frog : 100 %, Bovine : 100 %, Chicken : 100 %, Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Zebrafish : 100 %
Characteristics This is a rabbit polyclonal antibody against RPL3. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name RPL3
Background Ribosomes, the complexes that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL3 is a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L3P family of ribosomal proteins and it is located in the cytoplasm. The protein can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation. Ribosomes, the complexes that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L3P family of ribosomal proteins and it is located in the cytoplasm. The protein can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation. This gene is co-transcribed with several small nucleolar RNA genes, which are located in several of this gene's introns. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Molecular Weight 46 kDa
Gene ID 6122
NCBI Accession NP_000958, NM_000967
UniProt P39023
Research Area Chromatin and Nuclear Signaling, Chromatin Binding Proteins, DNA/RNA
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 403 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Background publications Tu, Yan, Hood et al.: "Proteomics analysis of the interactome of N-myc downstream regulated gene 1 and its interactions with the androgen response program in prostate cancer cells." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 4, pp. 575-88, 2007 (PubMed).

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