Cytokeratin 8 (KRT8) antibody

Antigen: Cytokeratin 8 (KRT8)

Synonyms: K8, KO, CK8, CYK8, K2C8, CARD2, K18, CYK18, Card2, EndoA, Krt2-8, Krt-2.8, AA960620, AL022697, AU019895, MGC118110, KRT2-8

Clonality: Monoclonal (1B12)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN410935

Additional Information

Alternative name: keratin 8 (KRT8)

Gene ID: 3856

Immunogen: Recombinant fragment expressed in E.coli corresponding to amino acids 91-381 of human CK8

Format: Purified

Isotype: IgG2b  (Matching secondary antibodies)

Clone: 1B12

Molecular Weight: 53.5 kDa

Application Details

Application Notes: Western blot: 1:5000, IF (1:100)

Concentration: 0.5-1.0 mg/ml (Lot dependent)

Purity: Purified

Purification: Purified from mouse ascites fluids by affinity chromatography

Buffer: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Restrictions: For Research Use only

Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT8(RC209570) (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT8. CK8 antibody (1B12 ) at 1:5000 dilution + Hela cell lysate IF Image Anti-KRT8 mouse monoclonal antibody (ABIN410935) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KRT8(RC209570). IF Image Immunofluorescent staining of HeLa cells using anti-KRT8 mouse monoclonal antibody (ABIN410935).

anti-Cytokeratin 8 (KRT8) antibody (Image 2)

Anti-KRT8 mouse monoclonal antibody (ABIN410935) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KRT8(RC209570). Immunofluorescent staining of HeLa cells using anti-KRT8 mouse monoclonal antibody (ABIN410935). Immunofluorescent staining of A549 cells using anti-KRT8 mouse monoclonal antibody (ABIN410935).