PDIA3 antibody

Catalog No. ABIN452267

The Mouse Monoclonal anti-PDIA3 antibody is suitable to detect PDIA3 in samples from Human, Mouse, Rat, Dog, Guinea Pig, Monkey, Cow, Rabbit, Pig and Hamster. It has been validated for WB, IP, IHC (p) and IHC (fro).

Quick Overview for PDIA3 antibody (ABIN452267)

Target: PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Monkey, Cow, Rabbit, Pig, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This PDIA3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: MaP-Erp57

Product Details anti-PDIA3 Antibody

Specificity: This antibody detects ERp57 / GRP58 at ~57 kDa.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Bovine, Canine, Guinea pig, Hamster, Monkey, Pig, Rabbit, Rat

Characteristics: Synonyms: Protein disulfide-isomerase A3, ERP57, ERP60, GRP58, Protein Disulfide Isomerase, ERmarker, Disulfide isomerase ER-60

Purification: Protein G Chromatography

Immunogen: Human recombinant ERp57 (GRP58)

Isotype: IgG1

Application Details

Application Notes: Western blot (8): 1: 2000 (ECL). Immunoprecipitation (9). Immunohistochemistry on frozen sections (8).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS pH 7.4, 50 % Glycerol, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for PDIA3

Target: PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))

Alternative Name: PDIA3

Background: ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glucosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface (6). ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus (7).Synonyms: Disulfide isomerase ER-60, ER marker, ERP57, ERP60, GRP58, Protein Disulfide Isomerase, Protein disulfide-isomerase A3

Gene ID: 2923

UniProt: P30101

Pathways: Maintenance of Protein Location, Protein targeting to Nucleus, Cell RedoxHomeostasis