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PDIA3 antibody (Biotin)

PDIA3 Reactivity: Human WB, IHC, IF, IP, ICC Host: Mouse Monoclonal Map-ERp57 Biotin
Catalog No. ABIN2484406
  • Target See all PDIA3 Antibodies
    PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))
    Reactivity
    • 86
    • 38
    • 35
    • 8
    • 8
    • 7
    • 6
    • 6
    • 6
    • 6
    • 5
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 70
    • 28
    • 3
    Mouse
    Clonality
    • 66
    • 35
    Monoclonal
    Conjugate
    • 43
    • 7
    • 6
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This PDIA3 antibody is conjugated to Biotin
    Application
    • 78
    • 31
    • 28
    • 27
    • 18
    • 16
    • 15
    • 13
    • 13
    • 8
    • 4
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)
    Specificity
    Detects ~57 kDa.
    Cross-Reactivity
    Cow, Dog, Guinea Pig, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat
    Purification
    Protein G Purified
    Immunogen
    Human recombinant ERp57 (Grp58)
    Clone
    Map-ERp57
    Isotype
    IgG1
    Top Product
    Discover our top product PDIA3 Primary Antibody
  • Application Notes
    • WB (1:2000)
    • IHC (1:100)
    • ICC/IF (1:100)
    • optimal dilutions for assays should be determined by the user.
    Comment

    0.5 μg/ml of ABIN2484406 was sufficient for detection of ERp57 in 10 μg of heat shock Heal Lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Conjugated antibodies should be stored at 4°C
  • Target
    PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))
    Alternative Name
    ERp57 (PDIA3 Products)
    Synonyms
    ER60 antibody, ERp57 antibody, ERp60 antibody, ERp61 antibody, GRP57 antibody, GRP58 antibody, HsT17083 antibody, P58 antibody, PI-PLC antibody, grp-58 antibody, 58kDa antibody, Erp antibody, Grp58 antibody, PDI antibody, PDI-Q2 antibody, PLC[a] antibody, Plca antibody, 1 antibody, 25D3-membrane-associated antibody, sb:cb825 antibody, erp57 antibody, grp58 antibody, pdia3 antibody, protein disulfide isomerase family A member 3 antibody, protein disulfide-isomerase A3 antibody, protein disulfide isomerase associated 3 antibody, protein disulfide isomerase family A, member 3 antibody, protein disulfide isomerase family A member 3 S homeolog antibody, PDIA3 antibody, Tsp_10062 antibody, Pdia3 antibody, pdia3 antibody, pdia3.S antibody
    Background
    ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glycosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface (6). ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus (7).
    Gene ID
    2923
    NCBI Accession
    NP_005304
    UniProt
    P30101
    Pathways
    Maintenance of Protein Location, Protein targeting to Nucleus, Cell RedoxHomeostasis
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