TSPO antibody (C-Term)

Catalog No. ABIN452349

This anti-TSPO antibody is a Goat Polyclonal antibody detecting TSPO in WB and IHC (p). Suitable for Human.

Quick Overview for TSPO antibody (C-Term) (ABIN452349)

Target: TSPO (Peripheral-Type Benzodiazepine Receptor (TSPO))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TSPO antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TSPO Antibody

Binding Specificity: C-Term

Sequence: c-RDNHGWRGGR RLPE

Specificity: This antibody is expected to recognise one isoform of TSPO/PBR (NP_000705.2) only.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_000705.2. Genename: TSPO

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/128000. Western blot: 0.1-0.3 μg/mL. Approx 35 kDa band observed in Human Brain lysates(calculated MW of 18.5 kDa according to NP_000705.2). Immunohistochemistry: 2-4 μg/mL. In paraffin embedded Human Colon shows strongcytoplasm staining of absorptive cells.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for TSPO

Target: TSPO (Peripheral-Type Benzodiazepine Receptor (TSPO))

Alternative Name: TSPO / BZRP

Background: PBR (Peripheral type Benzodiazepine Receptor) is a ubiquitous protein (18 kDa) found in most steroidogenic tissues. It is located primarily in the outer mitochondrial membrane of steroid producing tissues and is found in association with a 34 kDa voltage dependent anion channel protein (VDAC). PBR interacts with some benzodiazepines and has different affinities than its endogenous counterpart. PBR appears to be a key factor in the transport of cholesterol from the outer to the inner mitochondrial membrane to permit the initiation of steroid hormone synthesis. It is thought to be part of the mitochondrial permeability transition pore (PT) and to be involved in apoptosis. It is speculated that patients with congenital lipoid adrenal hyperplasia, who cannot make any steroids, might have a genetic lesion in BZRP. A short form, PBRS is also expressed in the same tissues, but at a level about ten times that of PBR.Synonyms: MBR, Mitochondrial benzodiazepine receptor, PBR, PKBS, Peripheral-type benzodiazepine receptor, Translocator protein

Gene ID: 706

NCBI Accession: NP_000705

UniProt: P30536

Pathways: Cellular Response to Molecule of Bacterial Origin