HCN1 antibody (C-Term)

Catalog No. ABIN452405

Product Details anti-HCN1 Antibody

Target: HCN1 (Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 1 (HCN1))

Binding Specificity: AA 778-910, C-Term

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This HCN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody detects HCN1 cyclic nucleotidegated Channel at ~100 kDa. No cross reactivity against HCN2.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.

Characteristics: Synonyms: Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1, Braincyclic nucleotide-gated channel 1

Purification: Protein G Chromatography

Immunogen: Fusion protein amino acids 778-910 (cytoplasmic C-terminus) of rat HCN1 (also known aspotassium/sodium hyperpolarization-activated) cyclic nucleotide-gated channel

Clone: S70-28

Isotype: IgG1

Application Details

Application Notes: Western blot: 1 - 10 μg/mL (if results are off, try using the lysate without boiling. Immunoprecipitation. Immunohistochemistry: 0.1 - 1.0 μg/mL (Perox).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS pH 7.4 containing 50 % Glycerol and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for HCN1

Target: HCN1 (Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 1 (HCN1))

Alternative Name: HCN1 / BCNG1 (HCN1 Products)

Synonyms: BCNG-1 antibody, BCNG1 antibody, HAC-2 antibody, Bcng1 antibody, C630013B14Rik antibody, HAC2 antibody, hyperpolarization activated cyclic nucleotide gated potassium channel 1 antibody, hyperpolarization-activated cyclic nucleotide-gated potassium channel 1 antibody, hyperpolarization-activated, cyclic nucleotide-gated K+ 1 antibody, hcn1 antibody, HCN1 antibody, Hcn1 antibody

Background: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand- gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels, each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). Specifically, hyperpolarization-activated cation channels of the HCN gene family, such as HCN1, play a crucial role in the regulatons of cell excitability. Importantly, they contribute to spontaneous rhythmic activity in both the heart and brain (3).Synonyms: Brain cyclic nucleotide-gated channel 1, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1

Gene ID: 84390

UniProt: Q9JKB0

Pathways: Asymmetric Protein Localization