Reactive to serine133 phosphorylated Human CREB peptide fragment, and reactive to KLH or BSA conjugated Serine133 phosphorylated Human CREB peptide fragment. Not reactive to un-conjugated KLH or BSA.
Cross-Reactivity (Details)
Species reactivity (tested):Human.
Characteristics
Synonyms: CREB-1, Cyclic AMP-responsive element-binding protein 1, cAMP-responsiveelement-binding protein 1
Purification
Affinity Chromatography on Protein G.
Immunogen
Phosphorylated peptide fragment of CREB (pSer133), conjugated with KLH
Indirect ELISA: The suggested antigen coating quantity is 1 μg/mL of peptide/or peptideconjugating in pH 9.5 Carbonate buffer, 100 μL/well. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore with Double distillated water to adjust the final concentration to 1.0 mg/mL.
Buffer
0.01 M PBS, pH 7.0 without preservatives.
Preservative
Without preservative
Storage
-20 °C
Storage Comment
Store the antibody at -20 °C. After reconstitution, aliquot and store at -20 °C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date
12 months
Target
CREB1
(cAMP Responsive Element Binding Protein 1 (CREB1))
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Background
Transcription factor CREB binds the cAMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth or neurotrophic factors. Protein kinase A and the calmodulin-dependent protein kinase CaMKII stimulate CREB phosphorylation at Ser133, a key regulatory site controlling transcriptional activity. Phosphorylation at Ser133 is also mediated by p44/42 MAP kinase, p90RSK, p38 MAP kinase and MSK1. CREB appears to play an important role in learning and memory in both flies and mice.Synonyms: CREB-1, Cyclic AMP-responsive element-binding protein 1, cAMP-responsive element-binding protein 1