SPRY1 antibody (C-Term)

Catalog No. ABIN452690

Product Details anti-SPRY1 Antibody

Target: SPRY1 (Sprouty Homolog 1, Antagonist of FGF Signaling (SPRY1))

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This SPRY1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects Sprouty at C-term.

Cross-Reactivity (Details): Species reactivity (tested):Human and Mouse.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CPSRGQGKPS, from the C Terminus of the protein sequence according to NP_005832.1, NP_955359.1.

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/16000. Western blot: 1-3 μg/mL (Successfully used in ERMS cells, PMID: 20068162). Immunohistochemistry: 1-3 μg/mL (Successfully used by anonymous customers in paraffinembedded Mouse Brain, showing nuclear staining).

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-SPRY1 antibody (ABIN452690)

Zhang, Shim, Wright, Jurkevich, Khare: "Atypical role of sprouty in p21 dependent inhibition of cell proliferation in colorectal cancer." in: Molecular carcinogenesis, Vol. 55, Issue 9, pp. 1355-68, (2016) (PubMed).

Target Details for SPRY1

Target: SPRY1 (Sprouty Homolog 1, Antagonist of FGF Signaling (SPRY1))

Alternative Name: Sprouty Homolog 1 / SPRY1 (SPRY1 Products)

Synonyms: hSPRY1 antibody, SPRY1 antibody, LOC100223875 antibody, hspry1 antibody, xspry1 antibody, sprouty antibody, sprouty1 antibody, sprouty-1 antibody, Xtsprouty1 antibody, spry1a antibody, spry1b antibody, sprouty RTK signaling antagonist 1 antibody, sprouty RTK signaling antagonist 1 L homeolog antibody, sprouty RTK signaling antagonist 1 S homeolog antibody, sprouty homolog 1 (Drosophila) antibody, SPRY1 antibody, Spry1 antibody, spry1 antibody, spry1.L antibody, spry1.S antibody

Background: Members of the Sprouty family (Sprouty 1-4) are inducible negative regulators of growth factors that act through tyrosine kinase receptors. Mammalian Sprouty homologs share a well conserved cysteine-rich C-terminal domain with their Drosophilacounterpart. Both Sprouty 1 and 2 are anchored to membranes by palmitoylation, associate with caveolin-1 in perinuclear and vesicular structures and are phosphorylated on Serine residues. Upon stimulation, a subset is recruited to the leading edge of the plasma membrane. Sprouty 2 can associate with c-Cbl, a downregulator of RTK signaling, and inhibits the activities of several growth factors. Sprouty 2 also functions as a negative regulator of embryonic lung morphogenesis and growth. The wellconserved C-terminus of Sprouty contains two domains which are necessary for Sprouty 2 colocalization with microtubules and translocation to membrane ruffles. In addition, the C-terminus is required for the inhibition of cell migration and proliferation. In conclusion, members of Sprouty inhibit FGF and VEGF-mediated cell proliferation, suggesting that they may regulate angiogenesis in normal and disease processes.Synonyms: Sprouty 1, Spry-1

Gene ID: 10252

NCBI Accession: NP_005832

UniProt: O43609

Pathways: EGFR Signaling Pathway, EGFR Downregulation