Rabbit anti-Human Ig (N-Term), (Joining Chain) Antibody

Details for Product No. ABIN453127
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Antigen
Epitope
N-Term, Joining Chain
(172), (171), (145), (71), (20), (14), (10), (5), (4), (4), (3), (3), (2), (1), (1), (1)
Reactivity
Human
(358), (107), (43), (29), (18), (5), (5), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1)
Host
Rabbit
(243), (201), (93), (16), (15), (7), (3), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(74), (52), (50), (27), (22), (21), (20), (16), (12), (8), (4), (2), (2), (2), (2), (1), (1)
Application
ELISA, Western Blotting (WB)
Pubmed 2 references available
Quantity 0.4 mL
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Catalog No. ABIN453127
357.50 $
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Immunogen KLH conjugated synthetic peptide selected from the N-terminal region of human IGJ
Specificity This antibody reacts to IGJ.
Purification Saturated Ammonium Sulfate (SAS) precipitation
Background IGJ serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain- joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces larger polymers. It also help to bind these immunoglobulins to secretory component.
Alternate names: IGCJ, IGJ
Gene ID 3512
NCBI Accession NP_653247
UniProt P01591
Application Notes ELISA: 1/1,000. Western blotting: 1/50 - 1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (w/v) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
Expiry Date 12 months
Background publications Liu, Qian, Gritsenko et al.: "Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry." in: Journal of proteome research, Vol. 4, Issue 6, pp. 2070-80, 2005 (PubMed).

Ramachandran, Boontheung, Xie et al.: "Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry." in: Journal of proteome research, Vol. 5, Issue 6, pp. 1493-503, 2006 (PubMed).

Validation Images
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