TGFB1 antibody

Catalog No. ABIN453911

The Mouse Monoclonal anti-TGFB1 antibody is suitable to detect TGFB1 in samples from Human. It has been validated for EIA, IHC (fro) and Func.

Quick Overview for TGFB1 antibody (ABIN453911)

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TGFB1 antibody is un-conjugated

Application: Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Clone: 8C4

Product Details anti-TGFB1 Antibody

Specificity: Western blotting demonstrated that this TGF-beta antibody reacts to either dimeric (25 kDa) or monomeric (12.5 kDa) molecules of TGF-beta under non-reducing and reducing condition respectively. Recognizes both Human platelet-derived and recombinant TGF beta in ELISA.

Purification: Affinity Chromatography on Protein G.

Immunogen: TGF-beta from Human platelets.

Isotype: IgG1

Application Details

Application Notes: ELISA. Neutralizing Activity: This MAb neutralizes TGF beta activity in vitro in inhibition assay ofCCL/64 cell growth and also neutralize the growth promoting action of TGF-beta in theNRK-49F colony forming assays. The effect of micro-injection of this MAb into oneblastomere of two cell stage Xenopus embryos indicated that this MAb was able toneutralize effectively the bioactivity of TG beta in vivo. Immunohistochemistry: May be used in immunohistochemical techniques to locateTGF-beta within tissues.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with Double distillated water to adjust the final concentration to 1.00 mg/mL.

Buffer: 0.01 M PBS, pH 7.0 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for TGFB1

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Alternative Name: TGFB1

Background: TGF beta 1 (Transforming Growth Factor Beta 1) is a multifunctional peptide capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types. Transformed as well as non neoplastic tissues, release transforming growth factors, and essentially all cells possess a specific TGF beta 1 receptor. The multimodal nature of TGF beta 1 is seen in its ability to stimulate or inhibit cellular proliferation. TGF beta 1 plays a fundamental role in tissue growth and differentiation involvement in adipogenesis, myogenesis, chondrogenesis, osteogenesis, epithelial cell differentiation and immune cell function. The C-terminal 112 amino acids constitute the mature protein.Synonyms: TGF-beta-1, TGFB, Transforming growth factor beta-1

Gene ID: 7040

NCBI Accession: NP_000651

UniProt: P01137

Pathways: EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy, Cancer Immune Checkpoints