TGFB1 antibody (Middle Region)

Catalog No. ABIN2780306

The Rabbit Polyclonal anti-TGFB1 antibody (ABIN2780306) specifically detects TGFB1 in WB, IHC and IF. The antibody is reactive with Rat, Mouse, Pig, Cow, Guinea Pig, Dog, Sheep, Horse and Goat samples.

Quick Overview for TGFB1 antibody (Middle Region) (ABIN2780306)

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Reactivity: Rat, Mouse, Pig, Cow, Guinea Pig, Dog, Sheep, Horse, Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TGFB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-TGFB1 Antibody

Binding Specificity: Middle Region

Purpose: TGFB1 Antibody - middle region

Sequence: DTPEWLSFDV TGVVRQWLNQ GDGIQGFRFS AHCSCDSKDN KLHVEINGIS

Predicted Reactivity: Cow: 79%, Dog: 79%, Goat: 79%, Guinea Pig: 87%, Horse: 79%, Mouse: 100%, Pig: 93%, Rat: 93%, Sheep: 79%

Characteristics: This is a rabbit polyclonal antibody against TGFB1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of mouse TGFB1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP37894-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for TGFB1

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Alternative Name: TGFB1

Background:

Background Information: Tgfb1 is a multifunctional protein that control proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGFB1 and essentially all of them have specific receptors for this protein. It regulates the actions of many other growth factors and determines a positive or negative direction of their effects. It plays an important role in bone remodelling. It is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.

Gene Name: Transforming growth factor, beta 1

Alternative Symbols: Tgfb, Tgfb-1, TGFbeta, TGF-beta, TGFbeta1, TGF-beta1

Protein Name: Transforming growth factor beta-1

Molecular Weight: 43kDa

Gene ID: 21803

NCBI Accession: NP_035707

UniProt: P04202

Pathways: EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy, Cancer Immune Checkpoints