HSF1 antibody

Catalog No. ABIN453930

Product Details anti-HSF1 Antibody

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Monkey, Guinea Pig, Hamster

Host: Rat

Clonality: Monoclonal

Conjugate: This HSF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Electrophoretic Mobility-Shift Assay (EMSA)

Specificity: This antibody detects an ~85 kDa protein in unstressed cell lysates, and an ~95 kDa protein in heat shocked cell lysates, corresponding to the molecular mass of inactive and active forms of HSF1 on SDS-PAGE immunoblots.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Rat, Bovine, Guinea pig, Hamster, Monkey, Rabbit

Characteristics: Synonyms: HSTF1, HSF-1, Heat shock transcription factor 1

Purification: Purified

Immunogen: Purified recombinant HSF1 protein

Clone: 10H8

Isotype: IgG1

Application Details

Application Notes: Western blot (see ref.1). Immunoprecipitation. Immunocytochemistry (see ref.1). ELISA (see ref.1). Gel Mobility Shift Assay (see ref.1).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.09 % sodium azide in 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C. Avoid repeated freezing andthawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for HSF1

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Alternative Name: Heat Shock Factor 1 / HSF1 (HSF1 Products)

Synonyms: hsf1 antibody, Hsf antibody, xhsf1 antibody, AA960185 antibody, HSTF1 antibody, HSF1 antibody, HSF antibody, HSF1c antibody, hsf1a antibody, hsf1b antibody, zHSF1a antibody, zHSF1b antibody, hstf1 antibody, heat shock factor antibody, Heat Shock Factor antibody, heat shock factor 1 antibody, heat shock transcription factor 1 antibody, heat shock transcription factor 1 S homeolog antibody, hsf1 antibody, hsf-1 antibody, Hsf1 antibody, HSF1 antibody, hsf1.S antibody

Background: HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the Nterminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transciptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).Synonyms: HSF-1, HSTF1, Heat shock transcription factor 1

Gene ID: 3297

UniProt: Q00613