SOD3 antibody

Catalog No. ABIN453955

Product Details anti-SOD3 Antibody

Target: SOD3 (Superoxide Dismutase 3, Extracellular (SOD3))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SOD3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects EC-SOD at ~35 kDa. Does not cross react with other SODs.

Cross-Reactivity (Details): Species reactivity (tested):Human, Rat, Mouse.

Characteristics: Synonyms: SOD-3, EC-SOD, Extracellular superoxide dismutase [Cu-Zn]

Purification: Protein G Chromatography

Immunogen: Human EC-SOD purified from aortas

Clone: 4G11G6

Isotype: IgG1

Application Details

Application Notes: Western blot: 1/1000.1 μg/mL was sufficient for detection of EC-SOD in 20 μg of Human cartillage lysate bycolorimetric Immunoblot using Goat anti-Mouse IgG (HRP) as the secondary antibodyELISA (7). Immunocytochemistry: 1/100. Immunohistochemistry on Frozen Sections: 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide and 50 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for SOD3

Target: SOD3 (Superoxide Dismutase 3, Extracellular (SOD3))

Alternative Name: Superoxide Dismutase 3 / SOD3 (SOD3 Products)

Synonyms: EC-SOD antibody, ECSOD antibody, SOD3 antibody, AI314465 antibody, LOC780439 antibody, fb05f10 antibody, si:dkey-117m1.6 antibody, sod3 antibody, wu:fb05f10 antibody, ECSODPT antibody, CuZnSOD antibody, SODA.4 antibody, superoxide dismutase 3 antibody, superoxide dismutase 3, extracellular antibody, superoxide dismutase 3, extracellular b antibody, superoxide dismutase 1 antibody, superoxide dismutase precursor ,putative antibody, SOD3 antibody, Sod3 antibody, sod3b antibody, sod3 antibody, Sod1 antibody, Smp_095980 antibody

Background: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2- to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2, 5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are three types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (6). SOD3 can also be distinguished by its heparin-binding capacity (1).Synonyms: EC-SOD, Extracellular superoxide dismutase [Cu-Zn], SOD-3

Gene ID: 6649

UniProt: P08294