Kallikrein 6 antibody (C-Term)

Catalog No. ABIN453970

This Goat Polyclonal antibody specifically detects Kallikrein 6 in WB and EIA. It exhibits reactivity toward Mouse, Rat and Cow.

Quick Overview for Kallikrein 6 antibody (C-Term) (ABIN453970)

Target: Kallikrein 6 (KLK6)

Reactivity: Mouse, Rat, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This Kallikrein 6 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Kallikrein 6 Antibody

Binding Specificity: C-Term

Sequence: KEKPGVYTDV CTHIR

Cross-Reactivity (Details): Species reactivity (expected):Rat and Cow.
Species reactivity (tested):Mouse.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from the C-Terminus of Mouse Kallikrein-6 according to NP_035307.1.

Application Details

Application Notes: Peptide ELISA: Kallikrein-6 Antibody Detection limit dilution 1/16000. Western blot: 1-3 μg/mL. Approx 22 kDa band observed in Mouse Liver lysates (calculatedMW of 27.2 kDa according to NP_035307.1). An additional band of unknown identity wasalso consistently observed at 42 kDa. Both bands were successfully blocked by incubationwith the immunising peptide.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for Kallikrein 6

Target: Kallikrein 6 (KLK6)

Alternative Name: KLK6 / Kallikrein-6

Background: Tissue kallikreins are a family of extracellular serine proteases consisting of 15 members (1, 2). The genes of this family aligned in tandem on chromosome 19q13.4 represent the largest contiguous group of proteases within the human genome. Tissue kallikreins have attracted great interest as potential biomarkers for various cancers, including prostate, ovarian, breast, testicular, and lung (2, 3). Human Kallikrein 6 (hKLK6) is a member of tissue kallikrein family observed in breast and brain tissues, colon carcinoma cells, and oligodedrocytes (1 3). Known protein substrates of hKLK6 are myelin basic protein, the precursor of the Abeta amyloid peptide, and plasminogen. Its physiological functions may include the participation in demyelination processes as well as in the progression of inflammatory disease of the CNS. The level of hKLK6 is reduced in brain extracts of Alzheimer patients and increased in serum of patients with ovarian cancer.Synonyms: Neurosin, PRSS18, PRSS9, Protease M, SP59, Serine protease 18, Serine protease 9, Zyme

Gene ID: 5653

NCBI Accession: NP_001012982

UniProt: Q92876

Pathways: Complement System, Regulation of G-Protein Coupled Receptor Protein Signaling