Afamin antibody (Internal Region)

Catalog No. ABIN453974

Product Details anti-Afamin Antibody

Target: Afamin (AFM)

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This Afamin antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: Recognizes Afamin / AFM.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog) and Cow.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-NKDDRPKDLSLRE, from the internal region of Human AFM/Afamin according to NP_001124.1.

Application Details

Application Notes: Peptide ELISA: Afamin Antibody Detection limit dilution: 1/16000. Western blot: 0.3-1.0 μg/mL. Approx 80 kDa band observed in Human Brain (Cerebellumand Cerebral Cortex) lysates (calculated MW of 69.0 kDa according to NP_001124.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for Afamin

Target: Afamin (AFM)

Alternative Name: Afamin / AFM (AFM Products)

Synonyms: AFM antibody, ALB2 antibody, ALBA antibody, ALF antibody, Alf antibody, alpha-Alb antibody, afamin antibody, AFM antibody, Afm antibody

Background: AFM (afamin) is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These genes encode structurally related serum transport proteins that are thought to be evolutionarily related. AFM is regulated developmentally, expressed in the liver and secreted into the bloodstreamSynonyms: ALB2, ALBA, Alpha-albumin

Gene ID: 173

NCBI Accession: NP_001124

UniProt: P43652