Mouse anti-Human Bence-Jones Protein lambda (Chain lambda), (Free & Bound) Antibody
ELISA, WB
Monoclonal
NI 412 and NI 268
IgG1 kappa
unconjugated
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- Target
- Bence-Jones Protein lambda
- Binding Specificity
- Chain lambda, Free & Bound
- Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
- Application
- ELISA, Western Blotting (WB)
- Characteristics
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The reactivity of the this preparation of two monoclonal antibodies is restricted to polyclonal and monoclonal immunoglobulins of the lambda type, as well as free lambda light chains as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immuno-peroxidase staining. Monoclonal mouse antiserum to human Ig lambda chain, free and bound
- Purification
- Delipidated, heat inactivated, lyophilized, stable whole ascites.
- Immunogen
- Highly purified Bence Jones lambda proteins isolated from pooled human urine.
- Clone
- NI 412 and NI 268
- Isotype
- IgG1 kappa
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- Application Notes
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To identify the light chain type of immunoglobulins or free light chains in human serum, other body fluids, cell and tissue substrates and to determine its concentration in indirect techniques as ELISA, indirect immunoperoxidase staining and immunoblotting.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:200 upwards, in Western blotting from 1:400 upwards. These data should be interpreted as general recommendations only. - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.
- Concentration
- 1.0 mg/mL
- Buffer
- No foreign proteins added.
- Preservative
- Without preservative
- Handling Advice
- Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
- Storage
- 4 °C/-20 °C
- Storage Comment
- The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
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- Target
- Bence-Jones Protein lambda
- Alternative Name
- Bence Jones lambda
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