alpha 2 Macroglobulin antibody

Catalog No. ABIN457943

Product Details anti-alpha 2 Macroglobulin Antibody

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This alpha 2 Macroglobulin antibody is un-conjugated

Application: Immunoelectrophoresis (IEP)

Specificity: The antiserum does not cross-react with any other component of human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. of this antiserum has been tested in double radial immunodiffusion against several ser of other species with the following results: bovine + dog ± cat + goat - monkey ++ rabbit + mouse - rat -

Characteristics: Precipitating polyclonal goat antiserum to human alpha-2 macroglobulin

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogen: Alpha-2 macroglobulin(clotting factor I) is the major protein in the alpha-2 region of human serum after separation by immunoelectrophoresis. The concentration in normal plasma is about 2.5 mg/ml. It is a large polymer and a strong antigen. It forms about one third in the total of the alpha-2 globulins. The protein is isolated by ammonium sulphate precipitation, ion exchange chromatography, gelfiltration and preparative starch block electrophoresis. In the purification the activation of kallikrein is avoided, since it forms an irreversible bond with alpha-2 macroglobulin, thus contaminating the final immunogen. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: In precipitating techniques as immunoelectrophoresis and single or double radial immuno-diffusion (Mancini, Ouchterlony) to identify the presence of alpha-2 macroglobulin in human plasma or other body fluids or to determine its concentration. This antiserum is not intended for use in non-precipitating antibody binding or other highly sensitive assays, but this does exclude such use if proper controls are included.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. Antibody titre: Precipitin titre 1:64 when tested against pooled normal human plasma in agar-block immunodiffusion titration. No foreign proteins ad

Buffer: Delipidated, heat inactivated, lyophilized, stable whole antiserum

Preservative: Without preservative

Storage: 4 °C

Target Details for alpha 2 Macroglobulin

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Alternative Name: alpha-2 Macroglobulin (A2M Products)

Synonyms: A2MD antibody, CPAMD5 antibody, FWP007 antibody, S863-7 antibody, A2MAC1 antibody, A2m1 antibody, A2maa antibody, Mam antibody, A2mp antibody, A2M antibody, LOC733429 antibody, endod antibody, cpamd5 antibody, fwp007 antibody, s863-7 antibody, a2mb antibody, endodermin antibody, Alpha-2-M antibody, alpha-2-macroglobulin antibody, pregnancy-zone protein L homeolog antibody, alpha-2-macroglobulin S homeolog antibody, pregnancy zone protein antibody, alpha-2-macroglobulin-like antibody, A2M antibody, A2m antibody, LOC477699 antibody, pzp.L antibody, AZL_c00450 antibody, LOC100349077 antibody, a2m antibody, LOC100061656 antibody, LOC100090399 antibody, a2m.S antibody, LOC100469973 antibody, LOC100595735 antibody, PZP antibody, LOC100353095 antibody, LOC101122940 antibody, LOC101801552 antibody, LOC100911545 antibody

Background: The reactivity of the antiserum is restricted to human alpha-2 macroglobulin. In immunoelectro-phoresis and radial immunodiffusion, using various antiserum concentrations against normal human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified alpha-2 macroglobulin. No reaction is obtained with any other plasma protein components or serum

Pathways: Lipid Metabolism