Sheep anti-Mouse IgG2a(1a) (Fc Region) Antibody
ICC, ID, IEP
Polyclonal
IgG
unconjugated
-
- Target
- IgG2a(1a)
- Binding Specificity
- Fc Region
- Reactivity
- Mouse
-
Host
- Sheep
-
Clonality
- Polyclonal
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Application
- Immunocytochemistry (ICC), Immunodiffusion (ID), Immunoelectrophoresis (IEP)
- Specificity
- The reactivity of the antiserum is restricted to the Fc portion of the IgG2a molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against mouse serum of the allelic type Igh-1a, including Balb/C, CBA, AKR and NZB, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG2a(1a). No precipitation reaction is obtained with purified IgG2a(1b) (C57Bl and SJL/J), other IgG subclasses, IgA, IgG/Fab fragments or other serum proteinsPrecipitating polyclonal sheep antiserum to mouse IgG2a(1a), allotype specific
- Characteristics
-
Precipitating polyclonal sheep antiserum to mouse IgG2a(1a), allotype specific
Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal sheep serum. Antibody titre: Precipitin titre 1:32 when tested against pooled normal Igh-1a allotype mouse serum in agar-block immuno - Purification
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Delipidated, heat inactivated, lyophilized, stable whole antiserum
Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. - Immunogen
- Highly purified normal homogenous IgG2a(1a) isolated from mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
- Isotype
- IgG
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-
- Application Notes
- In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG2a (1a) in mouse serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of mouse IgG2a (1a) from serum or plasma. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such use labelled an unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity an performance requirements are available.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- No foreign proteins added.
- Preservative
- Without preservative
- Storage
- 4 °C
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- Target
- IgG2a(1a)
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