Insulin antibody (FITC)

Catalog No. ABIN458552

The Guinea Pig Polyclonal anti-Insulin antibody has been validated for IA. It is suitable to detect Insulin in samples from Pig.

Quick Overview for Insulin antibody (FITC) (ABIN458552)

Target: Insulin (INS)

Reactivity: Pig

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This Insulin antibody is conjugated to FITC

Application: Immunoassay (IA)

Product Details anti-Insulin Antibody (FITC)

Specificity: Inter-species cross-reactivity is a normal feature of antibodies to mammalian proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement. This antiserum fraction has not been tested for cross-reactivity.

Characteristics: Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal guinea pig antiserum to swine insulin

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG (7S) fraction by DEAE-chromatography.

Immunogen: Insulin is a hormone produced by the beta cells of the islets of Langerhans in the pancreas. It plays an important role in the metabolism of carbohydrate by its effects on the storage of glycogen in the liver, the conversion of carbohydrate to fat, and the oxidation of glucose by the peripheral tissues. Insulin has a molecular weight of 6,000 and contains two peptide chains linked by two disulphide bonds. The hormone is isolated from porcine pancreas. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: The immunoconjugate is intended for use in different types of highly sensitive fluorescence immunoassays on appropriately treated cell and tissue substrates. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used. Working dilutions are usually between 1:10 and 1:40.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: IgG protein concentration 10 mg/ml. Fluorochrome/IgG protein molar ratio (F/P) approximately 2.7. No foreign proteins added.

Buffer: Purified hyperimmune guinea pig IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile di stilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Target Details for Insulin

Target: Insulin (INS)

Alternative Name: Insulin

Background: The reactivity of the antiserum is directed to insulin as tested by ELISA and immunoblotting. No reaction was obtained with other swine serum proteins.

Pathways: NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation