Rabbit anti-Rat IgG2a (Fc Region) Antibody

Catalog No. ABIN458717

Product Details

Target: IgG2a

Binding Specificity: Fc Region

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Application: Immunodiffusion (ID), Immunoelectrophoresis (IEP)

Specificity: The reactivity of the antiserum is directed to the Fc portion of the subclass IgG2a.

Characteristics: Total protein and IgG concentration in the antiserum are comparable to those of pooled normal rabbit serum. Antibody titre: Precipitin titre not less then 1:32 when tested against pooled normal rat serum in agar block immunodiff

Purification: Delipidated, heat inactivated, lyophilized whole antiserum.
Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogen: Pools of purified homogenous IgG2a isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG2a in rat serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of rat IgG2a from serum or plasma.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized antiserum b y adding 1 mL sterile distilled water.

Buffer: No foreign proteins added.

Preservative: Without preservative

Handling Advice: Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4 °C, not ref rozen, and preferably used the same day.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Target Details

Target: IgG2a

Abstract: IgG2a Products

Target Type: Antibody