BHMT antibody (AA 391-402)

Catalog No. ABIN461951

Product Details anti-BHMT Antibody

Target: BHMT (Betaine--Homocysteine S-Methyltransferase (BHMT))

Binding Specificity: AA 391-402

Reactivity: Human, Rat, Monkey, Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: This BHMT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human BHMT.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Hamster, Panda (100%) Mouse, Dog, Bat, Bovine, Horse, Pig, Opossum, Chicken (92%) Rat, Elephant, Rabbit (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-EQQLKELFEKQK from the C-terminus of human BHMT (NP_001704.1 ). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Hamster, Panda (100%), Mouse, Dog, Bat, Bovine, Horse, Pig, Opossum, Chicken (92%), Rat, Elephant, Rabbit (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:16000), WB (0.03 - 0.1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for BHMT

Target: BHMT (Betaine--Homocysteine S-Methyltransferase (BHMT))

Alternative Name: BHMT (BHMT Products)

Synonyms: BHMT1 antibody, hm:zehn2153 antibody, wu:fb53h01 antibody, wu:fb63c08 antibody, wu:fj64d01 antibody, zgc:123027 antibody, betaine--homocysteine S-methyltransferase antibody, betaine--homocysteine S-methyltransferase L homeolog antibody, betaine-homocysteine methyltransferase antibody, betaine-homocysteine S-methyltransferase antibody, BHMT antibody, bhmt.L antibody, Bhmt antibody, bhmt antibody

Background: Name/Gene ID: BHMT

Synonyms: BHMT, BHMT1

Gene ID: 635

NCBI Accession: NP_001704

UniProt: Q93088

Pathways: Methionine Biosynthetic Process