NOVA1 antibody (AA 121-134)

Catalog No. ABIN461953

Product Details anti-NOVA1 Antibody

Target: NOVA1 (Neuro-Oncological Ventral Antigen 1 (NOVA1))

Binding Specificity: AA 121-134

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Bat, Chicken, Monkey, Pig, Xenopus laevis

Host: Goat

Clonality: Polyclonal

Conjugate: This NOVA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human NOVA1. This antibody is expected to recognise all three reported isoforms (NP_002506.2 , NP_006480.2 , NP_006482.1)

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Lizard, Xenopus (100%) Horse (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-REMPQNVAKTEPVS from an internal region of human NOVA1 (NP_002506.2 , NP_006480.2 , NP_006482.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Lizard, Xenopus (100%), Horse (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:64000), WB (0.01 - 0.03 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for NOVA1

Target: NOVA1 (Neuro-Oncological Ventral Antigen 1 (NOVA1))

Alternative Name: NOVA1 (NOVA1 Products)

Synonyms: Nova-1 antibody, NOVA1 antibody, si:ch211-222c22.9 antibody, 9430099M15Rik antibody, G630039L02 antibody, NOVA alternative splicing regulator 1 antibody, neuro-oncological ventral antigen 1 L homeolog antibody, neuro-oncological ventral antigen 1 antibody, RNA-binding protein Nova-1 antibody, lipid A export ATP-binding/permease protein antibody, NOVA1 antibody, Nova1 antibody, nova1.L antibody, nova1 antibody, LOC790874 antibody, novA1 antibody

Background: Name/Gene ID: NOVA1

Synonyms: NOVA1, Nova-1, Onconeural ventral antigen 1, RNA-binding protein Nova-1, Paraneoplastic Ri antigen

Gene ID: 4857

NCBI Accession: NP_002506, NP_006480, NP_006482

UniProt: P51513