NOVA1 antibody (Neuro-Oncological Ventral Antigen 1) (Internal Region)

Details for Product anti-NOVA1 Antibody No. ABIN334449
Target
Binding Specificity
Internal Region
12
4
4
3
2
2
1
1
1
1
1
1
1
Reactivity
Human
52
25
24
4
4
4
4
4
2
2
2
2
2
1
Host
Goat
39
7
6
Clonality
Polyclonal
Conjugate
This NOVA1 antibody is un-conjugated
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
36
19
13
6
6
5
4
1
1
Options
Purpose NOVA1
Immunogen Peptide with sequence C-REMPQNVAKTEPVS, from the internal region of the protein sequence according to NP_002506.2 , NP_006480.2 , NP_006482.1.
Blocking Peptide Blocking peptide for this product available: ABIN369762
Sequence REMPQNVAKT EPVS
Isotype IgG
Specificity This antibody is expected to recognise all three reported isoforms (NP_002506.2 , NP_006480.2 , NP_006482.1)
Predicted Reactivity Human
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Target
Alternative Name NOVA1 (NOVA1 Antibody Abstract)
Background NOVA1, neuro-oncological ventral antigen 1, Nova-1, paraneoplastic Ri antigen, ventral neuron-specific protein 1
Gene ID 4857
NCBI Accession NP_002506, NP_006480, NP_006482
Application Notes

DS WB Results: Approx 50-55 kDa band observed in Human Breast Cancer lysates (calculated MW of 51.7 kDa according to NP_002506.2). In transfected HEK293 transiently expressing NOVA1 a band of approx. 52 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.01-0.03 μg/mL. Primary incubation was 1 hour.

DS IHC Results: Paraffin embedded Human Brain (Cortex) and Heart. Recommended concentration: 5 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Images
Image no. 1 for anti-Neuro-Oncological Ventral Antigen 1 (NOVA1) (Internal Region) antibody (ABIN334449) ABIN334449 (5µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retri...
Image no. 2 for anti-Neuro-Oncological Ventral Antigen 1 (NOVA1) (Internal Region) antibody (ABIN334449) HEK293 overexpressing NOVA1 (ABIN5405914) and probed with ABIN334449 (mock transfecti...
Image no. 3 for anti-Neuro-Oncological Ventral Antigen 1 (NOVA1) (Internal Region) antibody (ABIN334449) ABIN334449 (0.01µg/ml) staining of Human Breast Cancer lysate (35µg protein in RIPA b...
Image no. 4 for anti-Neuro-Oncological Ventral Antigen 1 (NOVA1) (Internal Region) antibody (ABIN334449) ABIN334449 (5µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrie...