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DS WB Results: Approx 50-55 kDa band observed in Human Breast Cancer lysates (calculated MW of 51.7 kDa according to NP_002506.2). In transfected HEK293 transiently expressing NOVA1 a band of approx. 52 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.01-0.03 μg/mL. Primary incubation was 1 hour.
DS IHC Results: Paraffin embedded Human Brain (Cortex) and Heart. Recommended concentration: 5 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:64000.