MIA2 antibody
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- Target See all MIA2 Antibodies
- MIA2 (Melanoma Inhibitory Activity Protein 2 (MIA2))
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This MIA2 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA
- Specificity
- Human MIA-2
- Purification
- Immunoaffinity purified
- Immunogen
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E.coli derived recombinant Human MIA-2.
Type of Immunogen: Cell extract - Top Product
- Discover our top product MIA2 Primary Antibody
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- Application Notes
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Approved: ELISA, WB (0.1 - 0.2 μg/mL)
Usage: Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human pancreas infiltrating ductal adenocarcinoma tissue. The recommended concentration is 5 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Optimal results for these conditions were achieved without antigen retrieval. Optimal concentrations and conditions may vary. ELISA: To detect hMIA-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MIA-2 (LS-C104686) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMIA-2. Western Blot: To detect hMIA-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIA-2 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Sterile water
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.2
- Handling Advice
- Avoid freeze/thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- Target
- MIA2 (Melanoma Inhibitory Activity Protein 2 (MIA2))
- Alternative Name
- MIA2 (MIA2 Products)
- Synonyms
- MEA6 antibody, MGEA antibody, MGEA11 antibody, MGEA6 antibody, zgc:56047 antibody, melanoma inhibitory activity 2 antibody, CTAGE family, member 5 antibody, CTAGE family member 5, ER export factor antibody, MIA2 antibody, ctage5 antibody, CTAGE5 antibody, Mia2 antibody
- Background
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Name/Gene ID: MIA2
Family: MIA
Synonyms: MIA2, Melanoma inhibitory activity 2 - Gene ID
- 117153
- UniProt
- Q96PC5
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