TNFSF14 antibody (Biotin)
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- Target See all TNFSF14 Antibodies
- TNFSF14 (Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This TNFSF14 antibody is conjugated to Biotin
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Application
- Western Blotting (WB), ELISA
- Specificity
- Human LIGHT
- Purification
- Immunoaffinity purified
- Immunogen
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E.coli derived recombinant Human LIGHT.
Type of Immunogen: Cell extract - Top Product
- Discover our top product TNFSF14 Primary Antibody
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- Application Notes
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Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hLIGHT by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hLIGHT. To detect hLIGHT by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human LIGHT (LS-C104596) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hLIGHT. Western Blot: To detect hLIGHT by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLIGHT is 1.5-3 ng/lane, under either reducing or non-reducing conditions. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Sterile PBS containing 0.1 % BSA
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.2
- Handling Advice
- Avoid freeze/thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- Target
- TNFSF14 (Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))
- Alternative Name
- TNFSF14 / LIGHT (TNFSF14 Products)
- Synonyms
- TNFSF14 antibody, CD258 antibody, HVEML antibody, LIGHT antibody, LTg antibody, TR2 antibody, HVEM-L antibody, Ly113 antibody, TNF superfamily member 14 antibody, tumor necrosis factor (ligand) superfamily, member 14 antibody, TNFSF14 antibody, Tnfsf14 antibody
- Background
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Name/Gene ID: TNFSF14
Family: TNF
Synonyms: TNFSF14, CD258 antigen, CD258, Delta transmembrane LIGHT, Herpesvirus entry mediator A, HVEM-L, TR2, HVEML, LIGHT, LTg - Gene ID
- 8740
- UniProt
- O43557
- Pathways
- Cancer Immune Checkpoints
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