PPP2R1B antibody (AA 7-19)

Catalog No. ABIN479957

The Rabbit Polyclonal anti-PPP2R1B antibody is suitable to detect PPP2R1B in samples from Human, Mouse, Rat, Monkey, Pig, Cow, Horse, Rabbit, Dog, Zebrafish (Danio rerio), Xenopus laevis, Drosophila melanogaster, Hamster and Insect. It has been validated for WB, ICC and IP.

Quick Overview for PPP2R1B antibody (AA 7-19) (ABIN479957)

Target: PPP2R1B (Protein Phosphatase 2, Regulatory Subunit A, beta (PPP2R1B))

Reactivity: Human, Mouse, Rat, Monkey, Pig, Cow, Horse, Rabbit, Dog, Zebrafish (Danio rerio), Xenopus laevis, Drosophila melanogaster, Hamster, Insect

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPP2R1B antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-PPP2R1B Antibody

Binding Specificity: AA 7-19

Specificity: Recognizes human PP 2A/A (65kD). Species cross-reactivity: mouse, rat and bovine. Does not cross-react with other protein phosphatase isoforms.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bovine, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Opossum, Platypus, Xenopus, Salmon, Zebrafish, Stickleback, Seq squirt, Drosophila, Water flea, Mosquito, Ant, Nematode, Beetle, Capsaspora (100%).

Purification: Affinity purified

Immunogen: PP 2A/A peptide aa 7-19, DDSLYPIAVLIDE, conjugated to KLH. Peptide corresponds to the N-terminal peptide of PP 2A/A regulatory subunit having a MW of 65kD. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bovine, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Opossum, Platypus, Xenopus, Salmon, Zebrafish, Stickleback, Seq squirt, Drosophila, Water flea, Mosquito, Ant, Nematode, Beetle, Capsaspora (100%).

Isotype: IgG

Application Details

Application Notes: Approved: ICC (10 - 15 μg/mL), IP, WB (5 - 10 μg/mL)

Usage: Suitable for use in Western Blot, Immunocytochemistry, and Immunoprecipitation. Western Blot: 5-10 μg/mL. Immunocytochemistry: 10-15 μg/mL. Immunoprecipitation: Can be used in conjunction with anti-PP2A/C to immunoprecipitate heterotrimeric PP2A. Optimum dilutions to be determined by researcher.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.08 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Target Details for PPP2R1B

Target: PPP2R1B (Protein Phosphatase 2, Regulatory Subunit A, beta (PPP2R1B))

Alternative Name: PPP2R1B

Background: Name/Gene ID: PPP2R1B

Synonyms: PPP2R1B, PR65B, PP2A subunit A isoform R1-beta, PP2A-Abeta

Gene ID: 5519

UniProt: P30154

Pathways: PI3K-Akt Signaling, Mitotic G1-G1/S Phases, Hepatitis C, Toll-Like Receptors Cascades