MTOR antibody (pSer2481)

Catalog No. ABIN482059

This Rabbit Polyclonal antibody specifically detects MTOR in IHC, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MTOR antibody (pSer2481) (ABIN482059)

Target: MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MTOR antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-MTOR Antibody

Binding Specificity: AA 2447-2496, pSer2481

Specificity:

mTOR (Phospho-Ser2481) Antibody detects endogenous levels of mTOR only when phosphorylated at Ser2481.

PhosphorylationH:S2481 M:S2481 R:S2481

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human mTOR around the phosphorylation site of Ser2481.

Isotype: IgG

Application Details

Application Notes: IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000

Comment:

Unigene-Number: Hs.338207 (NCBI Gene Symbol: MTOR)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for MTOR

Target: MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))

Alternative Name: mTOR

Background:

Synonyms: FRAP, FRAP1, FRAP2, RAFT1, Rapamycin target protein, kinase mTOR

NCBI Gene Symbol: MTOR

Molecular Weight: 288 kDa

Gene ID: 2475

OMIM: 601231

UniProt: P42345

Pathways: PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, BCR Signaling, Warburg Effect