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Antibodies
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anti-Janus Kinase 2 (JAK2) (pTyr221) antibody
Janus Kinase 2 (JAK2) (pTyr221) antibody
| Antigen |
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| Synonyms |
JTK10, Fd17, C81284, AI504024, JAK2, DKFZp469G074 |
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Epitope
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
Western Blotting (WB) (36), Immunohistochemistry (IHC) (24), Immunofluorescence (IF) (21), Flow Cytometry (FACS) (18), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (12), ELISA (11), Enzyme Immunoassay (EIA) (8), Immunoprecipitation (IP) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Dot Blot (Dot) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN482495 |
| Quantity |
50µg (1 mg/ml) |
| Price |
150.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Alternative name
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JAK2 (Phospho-Tyr221)
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Gene ID
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3717 (NCBI Gene Symbol: JAK2)
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Swiss-Prot
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O60674
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Immunogen
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The antiserum was produced against synthesized peptide derived from human JAK2 around the phosphorylation site of Tyr221.
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Format
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Liquid
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Isotype
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IgG (Matching secondary antibodies)
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Description
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Synonyms: JAK-2, JAK2, Janus kinase 2, kinase Jak2
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Specificity
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JAK2 (Phospho-Tyr221) Antibody detects endogenous levels of JAK2 only when phosphorylated at tyrosine221.
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Molecular Weight
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130 kDa
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Comments
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OMIM-Number: 147796 / 187950 / 254450 / 263300 / 600880 / 601626, Unigene-Number: Hs.656213
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Application Notes
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WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
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Concentration
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1 mg/ml
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Purity
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Greater 95%
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Purification
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
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Buffer
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Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage
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Store at -20°C/1 year
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Restrictions
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For Research Use only
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Alternatives for antigen "Janus Kinase 2 (JAK2)", type "Antibodies"
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Hosts
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Rabbit (76), Mouse (4), Goat (2)
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Reactivities
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Human (74), Rat (Rattus) (59), Mouse (Murine) (51), Chicken (17), Cow (Bovine) (17), Dog (Canine) (16), Pig (Porcine) (16), Zebrafish (Brachydanio rerio) (1)
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Applications
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Western Blotting (WB) (36), Immunohistochemistry (IHC) (24), Immunofluorescence (IF) (21), Flow Cytometry (FACS) (18), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (12), ELISA (11), Enzyme Immunoassay (EIA) (8), Immunoprecipitation (IP) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Dot Blot (Dot) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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pTyr1007 (9), C-Term (3), pTyr221 (3), pTyr1007, pTyr1008 (1)
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