Histone H2B antibody (AA 10-59)

Catalog No. ABIN486155

The Rabbit Polyclonal anti-Histone H2B antibody is suitable to detect Histone H2B in samples from Human, Mouse and Monkey. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for Histone H2B antibody (AA 10-59) (ABIN486155)

Target: Histone H2B

Reactivity: Human, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H2B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Histone H2B Antibody

Binding Specificity: AA 10-59

Purpose: Histone H2B Antibody

Specificity: Histone H2B Polyclonal Antibody detects endogenous levels of Histone H2B protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Histone H2B. AA range:10-59

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Histone H2B

Target: Histone H2B

Background:

Synonyms: H2BFS, Histone H2B type F-S, Histone H2B.s, H2B/s

Background: H2BFS is a Pseudogene. Histone H2B type 1-H is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid. Diseases associated with H2BFS include endometrial stromal sarcoma. Among its related pathways are Packaging Of Telomere Ends. Gene Ontology (GO) annotations related to this gene include sequence-specific DNA binding and protein heterodimerization activity. An important paralog of this gene is HIST1H2BH.

Gene Name: H2BFS

Protein Name: Histone H2B type F-S

Molecular Weight: 13 kDa

Gene ID: 54145

UniProt: P57053