NFYB antibody (Middle Region)

Catalog No. ABIN486823

Product Details anti-NFYB Antibody

Target: NFYB (Nuclear Transcription Factor Y, beta (NFYB))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Cow, Pig, Rabbit, Guinea Pig, Horse, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFYB antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: EAMKGEKGIG GAVTATDGLS EELTEEAFTN QLPAGLITTD GQQQNVMVYT

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Yeast: 91%

Characteristics: This is a rabbit polyclonal antibody against NFYB. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NFYB

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 207 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NFYB

Target: NFYB (Nuclear Transcription Factor Y, beta (NFYB))

Alternative Name: NFYB (NFYB Products)

Synonyms: CBF-A antibody, CBF-B antibody, HAP3 antibody, NF-YB antibody, NFYB antibody, nfyb antibody, zgc:110533 antibody, zgc:110552 antibody, hap3 antibody, cbf-a antibody, cbf-b antibody, CBFB antibody, Nfya antibody, AA985999 antibody, Cbf-A antibody, nuclear transcription factor Y subunit beta antibody, nuclear transcription factor Y subunit beta L homeolog antibody, nuclear transcription factor Y, beta b antibody, nuclear transcription factor Y, beta a antibody, nuclear transcription factor-Y beta antibody, NFYB antibody, nfyb.L antibody, nfybb antibody, nfyba antibody, nfyb antibody, Nfyb antibody

Background: NFYB is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence, protein sequence alignments and experiments with mutants.
Alias Symbols: CBF-A, CBF-B, HAP3, NF-YB
Protein Interaction Partner: AGTRAP, POLE4, DRAP1, NFYA, LYN, CDK2, CCNE1, CCNA2, TP53, SMYD3, NFYC, MAOB, UBC, CREBBP, SP1, HDAC1, EP300, KAT2B, TP73, TAF12, TAF11, TAF6, GRB2, YBX3, CEBPZ, C1QBP, TBP, CIITA, CNTN2, MYC, ELF1, YBX1,
Protein Size: 207

Molecular Weight: 23 kDa

Gene ID: 4801

NCBI Accession: NM_006166, NP_006157

UniProt: P25208

Pathways: Regulation of Lipid Metabolism by PPARalpha