Mediator Complex Subunit 27 (MED27) (Middle Region) antibody

Details for Product No. ABIN487113
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Antigen
Synonyms
CG1245, CRSP34, Dmel\\CG1245, Med27, Trap37, dCRSP34, dCrsp34, dTRAP37, med27, MED27, CRSP8, DDBDRAFT_0216764, DDBDRAFT_0266847, DDB_0216764, DDB_0266847, DKFZp459I1912, crsp8, zgc:66302, CRAP34, TRAP ... show more
CG1245, CRSP34, Dmel\\CG1245, Med27, Trap37, dCRSP34, dCrsp34, dTRAP37, med27, MED27, CRSP8, DDBDRAFT_0216764, DDBDRAFT_0266847, DDB_0216764, DDB_0266847, DKFZp459I1912, crsp8, zgc:66302, CRAP34, TRAP37, 1500015J03Rik, 2310042P07Rik, AA682045, Crsp8, D2Ertd434e, RGD1564993 show less
Epitope
Middle Region
(8), (4), (3), (2), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Pig (Porcine), Xenopus laevis, Cow (Bovine), Dog (Canine), Human, Chicken
(22), (6), (1), (1), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(12), (10)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(22), (14), (3), (1)
Pubmed 1 reference available
Quantity 50 µg
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Catalog No. ABIN487113
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Immunogen Synthetic peptide directed towards the middle region of human MED27
Sequence LSRPNGTSAM LLVTLGKVLK VIVVMRSLFI DRTIVKGYNE NVYTEDGKLD
Predicted Reactivity Bovine : 100 %, Chicken : 100 %, Human : 100 %, Pig : 100 %, Mouse : 90 %, Rat : 90 %, Dog : 80 %
Characteristics This is a rabbit polyclonal antibody against MED27. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name MED27
Background The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. MED27 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.
Molecular Weight 35 kDa
Gene ID 9442
NCBI Accession NM_004269, NP_004260
UniProt Q6P2C8
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 311 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Mediator Complex Subunit 27 (MED27) (Middle Region) antibody Host: Rabbit
Target Name: MED27
Sample Tissue: 293T
Antibody Dilution: 1.0 µg/mL MED27 is strongly supported by BioGPS gene expression data to be expressed in HEK293T
anti-Mediator Complex Subunit 27 (MED27) (Middle Region) antibody (2) Host: Rabbit
Target Name: MED27
Sample Tissue: 721_B
Antibody Dilution: 1.0 µg/mL MED27 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells
anti-Mediator Complex Subunit 27 (MED27) (Middle Region) antibody (3) Host: Rabbit
Target Name: MED27
Sample Tissue: Hela
Antibody Dilution: 1.0 µg/mL MED27 is strongly supported by BioGPS gene expression data to be expressed in HeLa
Background publications Humphray, Oliver, Hunt et al.: "DNA sequence and analysis of human chromosome 9. ..." in: Nature, Vol. 429, Issue 6990, pp. 369-74, 2004 (PubMed).

Validation Images
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