Mediator Complex Subunit 27 (MED27) (Middle Region) antibody

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Antigen
Synonyms
CG1245, CRSP34, Dmel\\CG1245, Med27, Trap37, dCRSP34, dCrsp34, dTRAP37, med27, MED27, CRSP8, DDBDRAFT_0216764, DDBDRAFT_0266847, DDB_0216764, DDB_0266847, DKFZp459I1912, crsp8, zgc:66302, CRAP34, TRAP ... show more
CG1245, CRSP34, Dmel\\CG1245, Med27, Trap37, dCRSP34, dCrsp34, dTRAP37, med27, MED27, CRSP8, DDBDRAFT_0216764, DDBDRAFT_0266847, DDB_0216764, DDB_0266847, DKFZp459I1912, crsp8, zgc:66302, CRAP34, TRAP37, 1500015J03Rik, 2310042P07Rik, AA682045, Crsp8, D2Ertd434e, RGD1564993 show less
Epitope
Middle Region
(5), (4), (4), (3), (2), (1), (1)
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Xenopus laevis
(22), (8), (2), (2), (2), (2), (1), (1), (1), (1)
Host
Rabbit
(14), (8)
Clonality
Polyclonal
Application
Western Blotting (WB)
(22), (9), (1), (1)
Pubmed 1 reference available
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Quantity 50 μg
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Immunogen Synthetic peptide directed towards the middle region of human MED27
Blocking Peptide Blocking peptide for this product available: Mediator Complex Subunit 27 (MED27) (Middle Region) peptide (ABIN979950)
Sequence LSRPNGTSAM LLVTLGKVLK VIVVMRSLFI DRTIVKGYNE NVYTEDGKLD
Predicted Reactivity Bovine : 100 %, Chicken : 100 %, Human : 100 %, Pig : 100 %, Mouse : 90 %, Rat : 90 %, Dog : 80 %
Characteristics This is a rabbit polyclonal antibody against MED27. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name MED27 (MED27 Antibody Abstract)
Background The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. MED27 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.
Molecular Weight 35 kDa
Gene ID 9442
NCBI Accession NM_004269, NP_004260
UniProt Q6P2C8
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 311 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-Mediator Complex Subunit 27 (MED27) (Middle Region) antibody (ABIN487113) Host: Rabbit
Target Name: MED27
Sample Tissue: 293T
Antibody Dil...
Western Blotting (WB) image for anti-Mediator Complex Subunit 27 (MED27) (Middle Region) antibody (ABIN487113) Host: Rabbit
Target Name: MED27
Sample Tissue: 721_B
Antibody Di...
Western Blotting (WB) image for anti-Mediator Complex Subunit 27 (MED27) (Middle Region) antibody (ABIN487113) Host: Rabbit
Target Name: MED27
Sample Tissue: Hela
Antibody Dil...
Background publications Humphray, Oliver, Hunt et al.: "DNA sequence and analysis of human chromosome 9. ..." in: Nature, Vol. 429, Issue 6990, pp. 369-74, 2004 (PubMed).

Catalog No. ABIN487113
$289.00
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