Cholesterol Esterase antibody (C-Term)

Catalog No. ABIN487123

This Rabbit Polyclonal antibody specifically detects Cholesterol Esterase in WB. It exhibits reactivity toward Human, Mouse, Rat, Horse and Pigand has been mentioned in 1 publication.

Quick Overview for Cholesterol Esterase antibody (C-Term) (ABIN487123)

Target: Cholesterol Esterase (CEL)

Reactivity: Human, Mouse, Rat, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cholesterol Esterase antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Cholesterol Esterase Antibody

Binding Specificity: C-Term

Sequence: VTPTGDSETA PVPPTGDSGA PPVPPTGDSE AAPVPPTDDS KEAQMPAVIR

Predicted Reactivity: Horse: 77%, Human: 100%, Mouse: 77%, Pig: 85%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against CEL. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C - terminal region of human CEL

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 756 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Cholesterol Esterase antibody (ABIN487123)

Panicot-Dubois, Thomas, Furie, Furie, Lombardo, Dubois: "Bile salt-dependent lipase interacts with platelet CXCR4 and modulates thrombus formation in mice and humans." in: The Journal of clinical investigation, Vol. 117, Issue 12, pp. 3708-19, (2007) (PubMed).

Target Details for Cholesterol Esterase

Target: Cholesterol Esterase (CEL)

Alternative Name: CEL

Background: CEL catalyzes fat and vitamin absorption. CEL acts in concert with pancreatic lipase and colipase for the complete digestion of dietary triglycerides.The protein encoded by this gene is a glycoprotein secreted from the pancreas into the digestive tract and from the lactating mammary gland into human milk. The physiological role of this protein is in cholesterol and lipid-soluble vitamin ester hydrolysis and absorption. This encoded protein promotes large chylomicron production in the intestine. Also its presence in plasma suggests its interactions with cholesterol and oxidized lipoproteins to modulate the progression of atherosclerosis. In pancreatic tumoral cells, this encoded protein is thought to be sequestrated within the Golgi compartment and is probably not secreted. This gene contains a variable number of tandem repeat (VNTR) polymorphism in the coding region that may influence the function of the encoded protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: BAL, BSDL, BSSL, CELL, CEase, FAP, FAPP, LIPA, MODY8
Protein Interaction Partner: OLR1, BPGM, LTF,
Protein Size: 756

Molecular Weight: 80 kDa

Gene ID: 1056

NCBI Accession: NM_001807, NP_001798

UniProt: P19835

Pathways: Lipid Metabolism