Cholesterol Esterase antibody (Internal Region)

Catalog No. ABIN7219050

The Rabbit Polyclonal anti-Cholesterol Esterase antibody (ABIN7219050) specifically detects Cholesterol Esterase in ELISA, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Cholesterol Esterase antibody (Internal Region) (ABIN7219050)

Target: Cholesterol Esterase (CEL)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cholesterol Esterase antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Cholesterol Esterase Antibody

Binding Specificity: Internal Region

Purpose: Rabbit Anti-CEL Polyclonal Antibody

Specificity: CEL Polyclonal Antibody detects endogenous levels of CEL protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human CEL

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Cholesterol Esterase

Target: Cholesterol Esterase (CEL)

Alternative Name: CEL

Background: CEL, BAL, Bile salt-activated lipase, BAL, Bile salt-stimulated lipase, BSSL, Bucelipase, Carboxyl ester lipase, Cholesterol esterase, Pancreatic lysophospholipase, Sterol esteraseThe carboxyl ester lipase encoded by CEL is a glycoprotein secreted from the pancreas into the digestive tract and from the lactating mammary gland into human milk. The physiological role of this protein is in cholesterol and lipid-soluble vitamin ester hydrolysis and absorption. This encoded protein promotes large chylomicron production in the intestine. Also its presence in plasma suggests its interactions with cholesterol and oxidized lipoproteins to modulate the progression of atherosclerosis. In pancreatic tumoral cells, this encoded protein is thought to be sequestrated within the Golgi compartment and is probably not secreted. This gene contains a variable number of tandem repeat (VNTR) polymorphism in the coding region that may influence the function of the encoded protein.

Gene ID: 1056

UniProt: P19835

Pathways: Lipid Metabolism