Verified
ACAT1
Reactivity: Human, Mouse, Rat
WB, ELISA, IF
Host: Goat
Polyclonal
unconjugated
Application Notes
Peptide ELISA: 1/2000. Western blot: Approx 45 kDa band observed in Human, Mouse and Rat Liver lysates(calculated MW of 45.5 kDa according to Human NP_000010.1 and 44.85 kDa to MouseNP_659033.1). Recommended concentration: 0.1 - 0.3 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
ACAT1, acetoacetyl CoA thiolase, plays a major role in ketone body metabolism. It is a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in the gene encoding ACAT1 are associated with the alpha-methylacetoaceticaciduria disorder,an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.Synonyms: Acetoacetyl-CoA thiolase T2, Acetyl-CoA acetyltransferase mitochondrial