ATP6V0D2 antibody (epsilon subunit)

Catalog No. ABIN488577

The Goat Polyclonal anti-ATP6V0D2 antibody (ABIN488577) specifically detects ATP6V0D2 in WB and IF. The antibody is reactive with Arabidopsis thaliana, Nicotiana tabacum, Tomato, Avena strigosa, Oryza sativa and Alfalfa samples.

Quick Overview for ATP6V0D2 antibody (epsilon subunit) (ABIN488577)

Target: ATP6V0D2 (ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2))

Reactivity: Arabidopsis thaliana, Nicotiana tabacum, Tomato, Avena strigosa, Oryza sativa, Alfalfa

Host: Goat

Clonality: Polyclonal

Conjugate: This ATP6V0D2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-ATP6V0D2 Antibody

Binding Specificity: epsilon subunit

Purpose: Anti-V-ATPase | Epsilon subunit of tonoplast H+ATPase (affinty purified, goat antibody)

Cross-Reactivity (Details): Arabidopsis thaliana, Avena strigosa, Medicago truncatula, Nicotiana tabacum, Oryza sativa, Solanum lycopersicum
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Algae,Chlamydomonas reinhardtii,Hordeum vulgare,Malus domestica,Mesembryanthemum sp.,Petunia sp.,Phaseolus sp.,Physcomitrium patens,Pteris vittata (fern),Ricinus communis,Thellungiella sp.,Zea mays,Vitis vinifera Bull frog,Chicken,Bovine,Drosophila melanogaster,Human,Mouse,Rat

Purification: Immunogen affinity purified serum

Immunogen: KLH-conjugated synthetic peptide chosen from subunit E of plant V-ATPase including Arabidopsis thaliana At4g11150. Peptide is conserved in vacuolar H+-ATPase subunit E

Application Details

Application Notes: 1: 600 (IF), 1 : 1000-1 : 3000 (WB)

Comment:

V-ATPase is very sensitive for the redox of the SDS buffer. We recommend using at least 50-100 mM DTT freshly prepared before handling the sample.2 hours incubation with primary antibody is recommended over over night incubation which can contribute to increased background.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details for ATP6V0D2

Target: ATP6V0D2 (ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2))

Alternative Name: V-ATPase

Background: Plant vacuole V-ATPase is responsible for energization of transport of ions and metabolites, and acts as well 'house-keeping' and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic sector. It is related to the FoF1 ATP synthase. Alternative protein names: Vacuolar proton pump subunit E, Protein EMBRYO DEFECTIVE 2448

Molecular Weight: 26 | 31 kDa (Arabidopsis thaliana)

Gene ID: 826716

UniProt: Q39258

Pathways: Transition Metal Ion Homeostasis, Proton Transport