Diazepam Binding Inhibitor antibody (AA 2-87)

Catalog No. ABIN4886558

Product Details anti-Diazepam Binding Inhibitor Antibody

Target: Diazepam Binding Inhibitor (DBI)

Binding Specificity: AA 2-87

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Diazepam Binding Inhibitor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Acyl-CoA-binding protein(DBI) detection. Tested with WB, IHC-P in Human.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Acyl-CoA-binding protein(DBI) detection. Tested with WB, IHC-P in Human.
Gene Name: diazepam binding inhibitor, acyl-CoA binding protein
Protein Name: Acyl-CoA-binding protein

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human DBI recombinant protein (Position: S2-I87). Human DBI shares 77.9% amino acid (aa) sequence identity with both mouse and rat DBI.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for Diazepam Binding Inhibitor

Target: Diazepam Binding Inhibitor (DBI)

Alternative Name: DBI (DBI Products)

Synonyms: ACBD1 antibody, ACBP antibody, CCK-RP antibody, EP antibody, dbib antibody, DBI antibody, wu:fb63e10 antibody, zgc:56108 antibody, zgc:77734 antibody, Acbp antibody, endozepine antibody, Acoabp3 antibody, Ep antibody, Odn antibody, RNACOABP3 antibody, Ttn antibody, acbd1 antibody, acbp antibody, cck-rp antibody, dbi antibody, dbi-a antibody, dbi-b antibody, dbia antibody, diazepam binding inhibitor, acyl-CoA binding protein antibody, diazepam binding inhibitor (GABA receptor modulator, acyl-CoA binding protein) S homeolog antibody, diazepam-binding inhibitor antibody, diazepam binding inhibitor (GABA receptor modulator, acyl-CoA binding protein) antibody, acyl-CoA-binding protein antibody, diazepam binding inhibitor antibody, diazepam binding inhibitor (GABA receptor modulator, acyl-CoA binding protein) L homeolog antibody, DBI antibody, dbi.S antibody, dbi antibody, LOC706367 antibody, Dbi antibody, dbi.L antibody

Background: Acyl-CoA-binding protein is a protein that in humans is encoded by the DBI gene. This gene encodes diazepam binding inhibitor, a protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid receptors located in brain synapses. The protein is conserved from yeast to mammals, with the most highly conserved domain consisting of seven contiguous residues that constitute the hydrophobic binding site for medium- and long-chain acyl-Coenzyme A esters. Diazepam binding inhibitor is also known to mediate the feedback regulation of pancreatic secretion and the postprandial release of cholecystokinin, in addition to its role as a mediator in corticotropin-dependent adrenal steroidogenesis. Three pseudogenes located on chromosomes 6, 8 and 16 have been identified. Multiple transcript variants encoding different isoforms have been described for this gene.

Synonyms: ACBD 1 | ACBD1 | ACBP | CCK RP | CCKRP | DBI | Endozepine | EP | P07108

Gene ID: 1622

UniProt: P07108